Preparation techniques alter the mineral and organic fractions of fish otoliths: insights using Raman micro-spectrometry

Type Article
Date 2013-05
Language English
Author(s) Jolivet Aurelie1, 2, Fablet Ronan3, 4, Bardeau Jean-Francois5, de Pontual HeleneORCID1
Affiliation(s) 1 : IFREMER, Dept Sci & Technol Halieut, ZI Pointe diable, F-29280 Plouzane, France.
2 : Univ Bretagne Occidentale, Inst Univ Europeen Mer, Lab Sci Environm Marin, UMR 6539, F-29280 Plouzane, France.
3 : Inst Telecom Telecom Bretagne, LabSTICC, UMR 3192, F-29238 Brest 03, France.
4 : Univ Europeenne Bretagne, F-35000 Rennes, France.
5 : LUNAM Univ, Inst Mol & Mat Mans, CNRS, UMR 6283, F-72085 Le Mans 09, France.
Source Analytical And Bioanalytical Chemistry (1618-2642) (Springer Heidelberg), 2013-05 , Vol. 405 , N. 14 , P. 4787-4798
DOI 10.1007/s00216-013-6893-2
WOS© Times Cited 8
Keyword(s) Epoxy resin, Otolith composition, Microstructure, Raman spectrometry
Abstract The high spatial resolution analysis of the mineral and organic composition of otoliths using Raman micro-spectrometry involves rigorous protocols for sample preparation previously established for microchemistry and trace elements analyses. These protocols often include otolith embedding in chemically neutral resin (i.e., resins which do not contain, in detectable concentration, elements usually sought in the otoliths). Such embedding may however induce organic contamination. In this paper, Raman micro-spectrometry reveals the presence of organic contamination onto the surface obtained from the use of epoxy resin, specifically Araldite. This contamination level varies depending on otolith structures. Core and checks, known as structural discontinuities, exhibit the most important level of contaminations. Our results suggest that otolith embedding with resin affects the organic matrix of the otolith, probably through an infiltration of the resin in the crystalline structure. The interpretation of chemical otolith signatures, especially Raman otolith signatures, and stable isotope analyses should then be revised in light of these results. In this respect, we propose a method for the correction of Raman otolith signatures for contamination effects.
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