Quantification of Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio cholerae in French Mediterranean coastal lagoons

Type Article
Date 2013-10
Language English
Author(s) Cantet Franck1, Hervio-Heath Dominique2, Caro A1, Le Mennec Cecile2, Monteil Caroline1, Quemere Catherine3, Jolivet-Gougeon Anne3, Colwell Rita R.4, Monfort Patrick1
Affiliation(s) 1 : Univ Montpellier 2&1, CNRS, IRD, IFREMER,UMR 5119, F-34095 Montpellier 05, France.
2 : IFREMER, RBE, EMP, Lab Microbiol LNR, F-29280 Plouzane, France.
3 : Univ Rennes 1, UPRES EA 1254 Microbiol, F-35000 Rennes, France.
4 : Univ Maryland, Ctr Bioinformat & Computat Biol, Inst Adv Comp Studies, College Pk, MD 20742 USA.
Source Research In Microbiology (0923-2508) (Elsevier Science Bv), 2013-10 , Vol. 164 , N. 8 , P. 867-874
DOI 10.1016/j.resmic.2013.06.005
WOS© Times Cited 42
Keyword(s) Vibrio, Lagoons, Shellfish, Water, Sediment, Human pathogen
Abstract Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio cholerae are human pathogens. Little is known about these Vibrio spp. in the coastal lagoons of France. The purpose of this study was to investigate their incidence in water, shellfish and sediment of three French Mediterranean coastal lagoons using the most probable number-polymerase chain reaction (MPN-PCR). In summer, the total number of V. parahaemolyticus in water, sediment, mussels and clams collected from the three lagoons varied from 1 to >1.1 x 10.3 MPN/l, 0.09 to 1.1 x 10.3 MPN/ml, 9 to 210 MPN/g and 1.5 to 2.1 MPN/g, respectively. In winter, all samples except mussels contained V. parahaemolyticus, but at very low concentrations. Pathogenic (tdh- or trh2-positive) V. parahaemolyticus were present in water, sediment and shellfish samples collected from these lagoons. The number of V. vulnificus in water, sediment and shellfish samples ranged from 1 to 1.1 x 10.3 MPN/l, 0.07 to 110 MPN/ml and 0.04 to 15 MPN/g, respectively, during summer. V. vulnificus was not detected during winter. V. cholerae was rarely detected in water and sediment during summer. In summary, results of this study highlight the finding that the three human pathogenic Vibrio spp. are present in the lagoons and constitute a potential public health hazard.
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Cantet Franck, Hervio-Heath Dominique, Caro A, Le Mennec Cecile, Monteil Caroline, Quemere Catherine, Jolivet-Gougeon Anne, Colwell Rita R., Monfort Patrick (2013). Quantification of Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio cholerae in French Mediterranean coastal lagoons. Research In Microbiology, 164(8), 867-874. Publisher's official version : https://doi.org/10.1016/j.resmic.2013.06.005 , Open Access version : https://archimer.ifremer.fr/doc/00148/25890/