Contribution to the understanding of the cycle of the protozoan parasite Marteilia refringens

Type Article
Date 2014-02
Language English
Author(s) Arzul IsabelleORCID1, Chollet Bruno1, Boyer S.2, Bonnet D.2, Gaillard Juliette1, Baldi Yoann3, Robert Maeva1, Joly Jean-Pierre1, Garcia Celine1, Bouchoucha Marc4
Affiliation(s) 1 : IFREMER SG2M LGPMM, Lab Genet & Pathol Marine Molluscs, F-17390 La Tremblade, France.
2 : Univ Montpellier 2, UMR5119, Lab EcoSym, F-34095 Montpellier 05, France.
3 : IFREMER Lab Environm & Resource Provence Azur Cor, F-20600 Bastia, France.
4 : IFREMER Lab Environm & Resource Provence Azur Cor, F-83507 La Seyne Sur Mer, France.
Source Parasitology (0031-1820) (Cambridge Univ Press), 2014-02 , Vol. 141 , N. 2 , P. 227-240
DOI 10.1017/S0031182013001418
WOS© Times Cited 17
Keyword(s) bivalves, Marteilia refringens, copepods, Paracartia latisetosa, zooplankton, parasite life cycle
Abstract The paramyxean parasite Marteilia refringens infects several bivalve species including European flat oysters Ostrea edulis and Mediterranean mussels Mytilus galloprovincialis. Sequence polymorphism allowed definition of three parasite types ‘M’, ‘O’ and ‘C’ preferably detected in oysters, mussels and cockles respectively.Transmission of the infection from infected bivalves to copepods Paracartia grani could be experimentally achieved but assays fromcopepods to bivalves failed. In order to contribute to the elucidation of the M. refringens life cycle, the dynamics of the infection was investigated in O. edulis, M. galloprovincialis and zooplankton over one year in Diana lagoon, Corsica (France). Flat oysters appeared non-infected while mussels were infected part of the year, showing highest prevalence in summertime. The parasite was detected by PCR in zooplankton particularly after the peak of prevalence in mussels. Several zooplanktonic groups including copepods, Cladocera, Appendicularia, Chaetognatha and Polychaeta appeared PCR positive. However, only the copepod species Paracartia latisetosa showed positive signal by in situ hybridization. Small parasite cells were observed in gonadal tissues of female copepods demonstrating for the first time that a copepod species other than P. grani can be infected with M. refringens. Molecular characterization of the parasite infecting mussels and zooplankton allowed the distinguishing of three Marteilia types in the lagoon.
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Arzul Isabelle, Chollet Bruno, Boyer S., Bonnet D., Gaillard Juliette, Baldi Yoann, Robert Maeva, Joly Jean-Pierre, Garcia Celine, Bouchoucha Marc (2014). Contribution to the understanding of the cycle of the protozoan parasite Marteilia refringens. Parasitology, 141(2), 227-240. Publisher's official version : https://doi.org/10.1017/S0031182013001418 , Open Access version : https://archimer.ifremer.fr/doc/00165/27635/