The effects of dietary carbohydrate sources and forms on metabolic response and intestinal microbiota in sea bass juveniles, Dicentrarchus labrax

Type Article
Date 2014-02
Language English
Author(s) Gatesoupe Joel1, Huelvan Christine2, Le Bayon Nicolas2, Severe Armelle2, Aasen Inga Marie3, Degnes Kristin F.3, Mazurais DavidORCID2, Panserat Stephane1, Zambonino-Infante Jose-LuisORCID2, Kaushik Sadasivam1
Affiliation(s) 1 : INRA, UR 1067, Nutrition, Métabolisme, Aquaculture, 64310 Saint-Pée-sur-Nivelle, France
2 : Ifremer, Unité de Physiologie Fonctionnelle des Organismes Marins, LEMAR UMR 6539, Ifremer, Centre de Brest, Laboratoire PFOM/ARN, BP 70, 29280 Plouzané, France
3 : SINTEF Materials and Chemistry, NO-7465 Trondheim, Norway
Source Aquaculture (0044-8486) (Elsevier), 2014-02 , Vol. 422-423 , P. 47-53
DOI 10.1016/j.aquaculture.2013.11.011
WOS© Times Cited 32
Keyword(s) Digestive enzymes, glucose metabolism, intestinal microbiota, viscerosomatic index, gene expression, metabolite analyses, vegetable feedstuffs
Abstract The aim of this work was to investigate the catabolic process of three kinds of dietary carbohydrates in the gut of sea bass juveniles, with the possible contribution of the intestinal microbiota to the nutrition of the host, and the subsequent effects on intermediary metabolism. A first diet contained waxy maize (99% amylopectin), a highly digestible form of starch. A second diet was less quickly digestible due to its high amylose content of resistant starch. Two other diets contained fibre instead of starch, either only cellulose as control, or also other non-starch polysaccharides brought by lupin meal. The effect of the diets on the host confirmed previous results, with the stimulation of glucose storage in the liver in sea bass fed the starchy diets, which caused a significant increase in liver weight, while lupin meal caused an increase in visceral mass. Glycaemia was higher 7 ± 1 h after the last meal in the group fed resistant starch, compared to the other dietary groups, while the fast digestion of waxy maize resulted already in hypertriglyceridemia, possibly due to hepatic neolipogenesis. At the same sampling time, the activity of free amylase was reduced in the intestine of sea bass fed resistant starch, but maltase activity was stimulated in the brush border membranes of enterocytes in the same group, confirming thus the timely digestion of resistant starch. Hepatic mRNA transcripts indicated that glucose metabolism was oriented towards neoglucogenesis by the high-fibre diets, and towards glucose storage by the starchy diets, especially with waxy maize. The diet influenced both faecal and mucosal microbiota, though in a different way, likely due to the interaction with the host. Lupin meal seemed potentially interesting as a source of prebiotic polysaccharides, by modifying the balance between Vibrio spp. and Clostridium sp. Both forms of starch were also partly metabolized by microbiota, resulting in an increased concentration of acetate in the faeces.
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Gatesoupe Joel, Huelvan Christine, Le Bayon Nicolas, Severe Armelle, Aasen Inga Marie, Degnes Kristin F., Mazurais David, Panserat Stephane, Zambonino-Infante Jose-Luis, Kaushik Sadasivam (2014). The effects of dietary carbohydrate sources and forms on metabolic response and intestinal microbiota in sea bass juveniles, Dicentrarchus labrax. Aquaculture, 422-423, 47-53. Publisher's official version : https://doi.org/10.1016/j.aquaculture.2013.11.011 , Open Access version : https://archimer.ifremer.fr/doc/00166/27763/