An Extended Network of Genomic Maintenance in the Archaeon Pyrococcus abyssi Highlights Unexpected Associations between Eucaryotic Homologs

Type Article
Date 2013-11
Language English
Author(s) Pluchon Pierre Francois1, 2, 3, Fouqueau Thomas4, Creze Christophe1, 2, 3, Laurent Sebastien1, 2, 3, Briffotaux Julien1, 2, 3, Hogrel Gaelle1, 2, 3, Palud Adeline1, 2, 3, Henneke Ghislaine1, 2, 3, Godfroy AnneORCID1, 2, 3, Hausner Winfried4, Thomm Michael4, Nicolas Jacques5, Flament Didier1, 2, 3
Affiliation(s) 1 : IFREMER, Lab Microbiol Environm Extremes, UMR6197, Plouzane, France.
2 : Univ Bretagne Occidentale, Lab Microbiol Environm Extremes, UMR6197, Plouzane, France.
3 : CNRS, Lab Microbiol Environm Extremes, UMR6197, Plouzane, France.
4 : Univ Regensburg, Lehrstuhl Mikrobiol, D-93053 Regensburg, Germany.
5 : IRISA INRIA, Rennes, France.
Source Plos One (1932-6203) (Public Library Science), 2013-11 , Vol. 8 , N. 11 , P. -
DOI 10.1371/journal.pone.0079707
WOS© Times Cited 10
Abstract In Archaea, the proteins involved in the genetic information processing pathways, including DNA replication, transcription, and translation, share strong similarities with those of eukaryotes. Characterizations of components of the eukaryotic-type replication machinery complex provided many interesting insights into DNA replication in both domains. In contrast, DNA repair processes of hyperthermophilic archaea are less well understood and very little is known about the intertwining between DNA synthesis, repair and recombination pathways. The development of genetic system in hyperthermophilic archaea is still at a modest stage hampering the use of complementary approaches of reverse genetics and biochemistry to elucidate the function of new candidate DNA repair gene. To gain insights into genomic maintenance processes in hyperthermophilic archaea, a protein-interaction network centred on informational processes of Pyrococcus abyssi was generated by affinity purification coupled with mass spectrometry. The network consists of 132 interactions linking 87 proteins. These interactions give insights into the connections of DNA replication with recombination and repair, leading to the discovery of new archaeal components and of associations between eucaryotic homologs. Although this approach did not allow us to clearly delineate new DNA pathways, it provided numerous clues towards the function of new molecular complexes with the potential to better understand genomic maintenance processes in hyperthermophilic archaea. Among others, we found new potential partners of the replication clamp and demonstrated that the single strand DNA binding protein, Replication Protein A, enhances the transcription rate, in vitro, of RNA polymerase. This interaction map provides a valuable tool to explore new aspects of genome integrity in Archaea and also potentially in Eucaryotes.
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Pluchon Pierre Francois, Fouqueau Thomas, Creze Christophe, Laurent Sebastien, Briffotaux Julien, Hogrel Gaelle, Palud Adeline, Henneke Ghislaine, Godfroy Anne, Hausner Winfried, Thomm Michael, Nicolas Jacques, Flament Didier (2013). An Extended Network of Genomic Maintenance in the Archaeon Pyrococcus abyssi Highlights Unexpected Associations between Eucaryotic Homologs. Plos One, 8(11), -. Publisher's official version : https://doi.org/10.1371/journal.pone.0079707 , Open Access version : https://archimer.ifremer.fr/doc/00171/28198/