Kosmotoga pacifica sp. nov., a thermophilic chemoorganoheterotrophic bacterium isolated from an East Pacific hydrothermal sediment
|Author(s)||L'Haridon Stephane1, 2, 3, Jiang Lijing4, Alain Karine1, 2, 3, Chalopin Morgane1, 2, 3, Rouxel Ouafae1, 2, 3, Beauverger Mikael1, 2, 3, Xu Hongxiu4, Shao Zongze4, Jebbar Mohamed1, 2, 3|
|Affiliation(s)||1 : Univ Bretagne Occidentale, IUEM, LMEE, UEB,UMR 6197, F-29280 Plouzane, France.
2 : CNRS, IUEM, LMEE, UMR 6197, F-29280 Plouzane, France.
3 : IFREMER, LMEE, UMR 6197, F-29280 Plouzane, France.
4 : SOA, Inst Oceanog 3, Key Lab Marine Biogenet Resources, Xiamen 361005, Peoples R China.
|Source||Extremophiles (1431-0651) (Springer Japan Kk), 2014-01 , Vol. 18 , N. 1 , P. 81-88|
|WOS© Times Cited||11|
|Keyword(s)||Thermotogales, Kosmotoga, Kosmotoga pacifica, Hydrothermal sediment|
|Abstract||A novel strictly anaerobic thermophilic heterotrophic bacterium, strain SLHLJ1T, was isolated from a Pacific hydrothermal sediment. Cells were Gram-negative coccobacilli (approximately 1.0 × 0.6 μm) with a toga. It grew at temperatures between 33 and 78 °C (optimum 70 °C). Elemental sulphur and l-cystine stimulated its growth. It contained C16:0, C16:1 ω11c, C18:0 and C18:1 ω9c as major fatty acids (>5 %), 3 phospholipids and 2 glycolipids as polar lipids. Its DNA G+C content was 43.7 mol%. Phylogenetic analyses based on 16S rRNA gene sequences placed strain SLHLJ1T within the family Thermotogaceae. The novel isolate was most closely related to Kosmotoga arenicorallina (97.93 % 16S rRNA gene sequence similarity), K. olearia (92.43 %) and K. shengliensis (92.17 %). On the basis of phenotypic, chemotaxonomic and phylogenetic comparisons with its closest relatives, we propose its assignment to a novel species of the genus Kosmotoga. The name Kosmotoga pacifica sp. nov. is proposed with strain SLHLJ1T (=DSM 26965T = JCM 19180T = UBOCC 3254T) as the type species.|