Phosphorus limitation might promote more toxin content in the marine invader dinoflagellate Alexandrium minutum

Alexandrium minutum (strain AM89BM) has been grown in semi-continuous culture (0.2 vol. d super(-1)) in N-limiting (NO sub(3) super(-): PO sub(4) super(3-)=1.6 and 3.16), in P-limiting (N: P=160 and 80), and in N and P balanced (N: P=16) media. The toxin content in cells changed greatly according to the N: P ratio. Cells grown in N: P balanced condition showed an average total paralytic shellfish poisoning (PSP) content of 1.24 fmol cell super(-1). In N-limiting conditions, cells contained ca. 3 times less toxin with mean values of 0.41-0.45 fmol cell super(-1). In contrast, cells grown in P-limiting conditions contained on average 3.5 and 7 times more toxins than in the balanced N: P condition: 4.31 fmol cell super(-1) in the N: P=160 medium and 8.01 fmol cell super(-1) in the N: P=80 medium. The toxin content per carbon unit shows the same trend; the lowest content in N: P<16 conditions was 6.5 fmol PSP nmol C super(-1), whereas in the N: P=16 condition it was 3.3 times higher at 21.8 fmol PSP nmol C super(-1) and 9 times higher in N: P>16 conditions at 59.0 fmol PSP nmol C super(-1). Since present trends in nutrient loading are assumed to have skewed some historically stable situations towards higher N: P ratios, the stimulation of toxin production by P-limiting media has important ecological consequences; in niches where DIN: DIP is >16, populations of A. minutum sufficiently concentrated to represent a significant fraction of shellfish food could pose a serious problem

Keyword(s)

Alexandrium minutum, Phosphorus limitation, Toxin content, PSP

How to cite
Maestrini Serge, Bechemin Christian, Grzebyk Daniel, Hummert Christian (2000). Phosphorus limitation might promote more toxin content in the marine invader dinoflagellate Alexandrium minutum. Plankton Biology & Ecology. 47 (1). 7-11. https://archimer.ifremer.fr/doc/00173/28422/

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