Localization and characterization of acid phosphatase activity in Bonamia ostreae (Ascetospora), an intrahemocytic protozoan parasite of the fIat oyster Ostrea edulis (Bivalvia)

Type Article
Date 1991-12
Language English
Author(s) Hervio Dominique1, Chagot D1, Godin P1, Grizel Henri1, Mialhe Eric1
Affiliation(s) 1 : IFREMER,UNITE RECH PATHOL IMMUNOL & GENET MOLEC,BIOL & ECOL INVERTEBRES MARINS LAB,BP 133,F-17390 LA TREMBLADE,FRANCE.
Source Diseases Of Aquatic Organisms (0177-5103) (Inter-research), 1991-12 , Vol. 12 , N. 1 , P. 67-70
DOI 10.3354/dao012067
WOS© Times Cited 31
Abstract Acid phosphatase activity was cytochemically detected at the ultrastructural level in the protozoan Bonamia ostreae, an intrahemocytic parasite of the flat oyster Ostrea edulis. Lead nitrate was used as capture agent for phosphate liberated during the hydrolysis of the substrate (cytidine 5' monophosphate). Lead phosphate precipitation, indicative of enzyme activity, was located in membrane-bound organelles corresponding to the cytoplasmic inclusions known as 'dense bodies'. Acid phosphatase activity level and sodium L-tartrate inhibition were measured spectrophotometrically from a purified parasite suspension. The possible involvement of this enzyme in intracellular survival of B. ostreae is discussed.
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Hervio Dominique, Chagot D, Godin P, Grizel Henri, Mialhe Eric (1991). Localization and characterization of acid phosphatase activity in Bonamia ostreae (Ascetospora), an intrahemocytic protozoan parasite of the fIat oyster Ostrea edulis (Bivalvia). Diseases Of Aquatic Organisms, 12(1), 67-70. Publisher's official version : https://doi.org/10.3354/dao012067 , Open Access version : https://archimer.ifremer.fr/doc/00182/29311/