Chronic dietary exposure to pyrolytic and petrogenic mixtures of PAHs causes physiological disruption in zebrafish - part I: Survival and growth

Type Article
Date 2014-12
Language English
Author(s) Vignet Caroline1, Le Menach Karyn2, Mazurais DavidORCID3, Lucas Julie1, 4, Perrichon Prescilla1, Le Bihanic Florane2, Devier Marie-Hélène2, Lyphout Laura1, 5, Frere Laura5, Begout Marie-LaureORCID5, Zambonino-Infante Jose Luis3, Budzinski Helene2, Cousin XavierORCID1, 6
Affiliation(s) 1 : IFREMER, Lab Ecotoxicol, F-17137 Lhoumeau, France.
2 : Univ Bordeaux 1, EPOC, UMR CNRS 5805, F-33405 Talence, France.
3 : IFREMER, Unit Funct Physiol Marine Organisms, UMR LEMAR 6539, F-29280 Plouzane, France.
4 : Inst Littoral & Environm, UMR Littoral Environm Soc LIENSs 7266, F-17000 La Rochelle, France.
5 : IFREMER, Lab Ressources Halieut, F-17137 Lhoumeau, France.
6 : INRA LPGP, F-35042 Rennes, France.
Source Environmental Science And Pollution Research (0944-1344) (Springer Heidelberg), 2014-12 , Vol. 21 , N. 24 , P. 13804-13817
DOI 10.1007/s11356-014-2629-x
WOS© Times Cited 17
Note PAHS AND FISH – EXPOSURE MONITORING AND ADVERSE EFFECTS – FROM MOLECULAR TO INDIVIDUAL LEVEL
Keyword(s) Danio rerio, Life cycle, Polycyclic aromatic hydrocarbon, Heavy oil, Light crude oil, Alkylated PAH, Jaw, Digestion
Abstract The release of polycyclic aromatic hydrocarbons (PAHs) into the environment has increased very substantially over the last decades leading to high concentrations in sediments of contaminated areas. To evaluate the consequences of long-term chronic exposure to PAHs, zebrafish were exposed, from their first meal at 5 days post fertilisation until they became reproducing adults, to diets spiked with three PAH fractions at three environmentally relevant concentrations with the medium concentration being in the range of 4.6–6.7 μg g−1 for total quantified PAHs including the 16 US-EPA indicator PAHs and alkylated derivatives. The fractions used were representative of PAHs of pyrolytic (PY) origin or of two different oils of differing compositions, a heavy fuel (HO) and a light crude oil (LO). Fish growth was inhibited by all PAH fractions and the effects were sex specific: as determined with 9-month-old adults, exposure to the highest PY inhibited growth of females; exposure to the highest HO and LO inhibited growth of males; also, the highest HO dramatically reduced survival. Morphological analysis indicated a disruption of jaw growth in larvae and malformations in adults. Intestinal and pancreatic enzyme activities were abnormal in 2-month-old exposed fish. These effects may contribute to poor growth. Finally, our results indicate that PAH mixtures of different compositions, representative of situations encountered in the wild, can promote lethal and sublethal effects which are likely to be detrimental for fish recruitment.
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Vignet Caroline, Le Menach Karyn, Mazurais David, Lucas Julie, Perrichon Prescilla, Le Bihanic Florane, Devier Marie-Hélène, Lyphout Laura, Frere Laura, Begout Marie-Laure, Zambonino-Infante Jose Luis, Budzinski Helene, Cousin Xavier (2014). Chronic dietary exposure to pyrolytic and petrogenic mixtures of PAHs causes physiological disruption in zebrafish - part I: Survival and growth. Environmental Science And Pollution Research, 21(24), 13804-13817. Publisher's official version : https://doi.org/10.1007/s11356-014-2629-x , Open Access version : https://archimer.ifremer.fr/doc/00188/29907/