Responses of the European flounder (Platichthys flesus) to a mixture of PAHs and PCBs in experimental conditions

Type Article
Date 2014-12
Language English
Author(s) Dupuy Celie1, 2, Galland Claire1, Devaux Alain3, 4, Bony Sylvie3, 4, Loizeau VeroniqueORCID5, Danion Morgane6, Pichereau Vianney1, Fournier Michel2, Laroche Jean1
Affiliation(s) 1 : Univ Bretagne Occidentale, Univ Europeenne Bretagne, Inst Univ Europeen Mer, UMR CNRS UBO IRD Ifremer 6523,Lab Sci Environm Ma, F-29280 Plouzane, France.
2 : Inst Natl Rech Sci, Inst Armand Frappier, Laval, PQ H7V 1B7, Canada.
3 : Univ Lyon, UMR LEHNA CNRS 5023, F-69100 Villeurbanne, France.
4 : INRA, USC IGH 1369, ENTPE, F-69518 Vaulx En Velin, France.
5 : IFREMER, Ctr Brest, Unite Biogeochim & Ecotoxicol, F-29280 Plouzane, France.
6 : ANSES, Agence Natl Securite Ssanit Alimentat Environm &, Lab Ploufragan Plouzane, F-29280 Plouzane, France.
Source Environmental Science And Pollution Research (0944-1344) (Springer Heidelberg), 2014-12 , Vol. 21 , N. 24 , P. 13789-13803
DOI 10.1007/s11356-014-2563-y
WOS© Times Cited 12
Keyword(s) Mixture of contaminants, Fish, Biomarker, Immunotoxicity, Detoxification process, DNA damage, PAHs, PCBs
Abstract A multibiomarker approach was developed to evaluate the juvenile European flounder responses to a complex mixture of 9 polycyclic aromatic hydrocarbons (PAHs) and 12 polychlorinated biphenyls (PCBs). Exposure was performed through contaminated food pellets displaying: (1) PAH and PCB levels similar to those detected in the heavily polluted Seine estuary, respectively in sediments and in flatfish and (2) ten times these concentrations. Several biomarkers of the immune system (e.g., lysozyme concentration and gene expression of complement component C3 and TNF-receptor), DNA damage (e.g., Comet assay), energetic metabolism (e.g., activity of cytochrome C oxidase), detoxification process (e.g., cytochrome P450 1A1 expression level: CYP1A1; betaine homocysteine methyl transferase expression level: BHMT) were investigated after 14 and 29 days of contamination, followed by a 14-days recovery period. After 29 days of contamination, the detoxification activity (CYP1A1 expression level) was positively correlated with DNA damages; the increase of the BHMT expression level could also be related to the detoxification process. Furthermore, after the recovery period, some biomarkers were still upregulated (i.e., CYP1A1 and BHMT expression levels). The immune system was significantly modulated by the chemical stress at the two concentration levels, and the lysozyme appeared to be the most sensitive marker of the mixture impact.
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Dupuy Celie, Galland Claire, Devaux Alain, Bony Sylvie, Loizeau Veronique, Danion Morgane, Pichereau Vianney, Fournier Michel, Laroche Jean (2014). Responses of the European flounder (Platichthys flesus) to a mixture of PAHs and PCBs in experimental conditions. Environmental Science And Pollution Research, 21(24), 13789-13803. Publisher's official version : https://doi.org/10.1007/s11356-014-2563-y , Open Access version : https://archimer.ifremer.fr/doc/00188/29914/