Monitoring of four DNA extraction methods upstream of high-throughput sequencing of Anisakidae nematodes
Different methods were evaluated to extract DNA from pooled nematodes belonging to Anisakis, Contracaecum, Pseudoterranova and Hysterothylacium genera isolated from edible fish. Pooled DNA extraction is the first and compulsory step to allow the identification of a large number of samples through high-throughput DNA sequencing with drastic time and cost reductions.
Keyword(s)
Anisakidae, High-throughput sequencing, Nematode, Pooled DNA extraction, Marine fish, COX2
Seesao Y., Audebert C., Verrez-Bagnis Veronique, Merlin S., Jerome Marc, Viscogliosi E., Dei-Cas E., Aliouat-Denis C. M., Gay Melanie (2014). Monitoring of four DNA extraction methods upstream of high-throughput sequencing of Anisakidae nematodes. Journal Of Microbiological Methods. 102. 69-72. https://doi.org/10.1016/j.mimet.2014.05.004, https://archimer.ifremer.fr/doc/00189/30020/
