Monitoring of four DNA extraction methods upstream of high-throughput sequencing of Anisakidae nematodes
Type | Article | ||||||||
---|---|---|---|---|---|---|---|---|---|
Date | 2014-07 | ||||||||
Language | English | ||||||||
Author(s) | Seesao Y.1, 2, Audebert C.3, 5, Verrez-Bagnis Veronique4, Merlin S.3, 5, Jerome Marc4, Viscogliosi E.2, Dei-Cas E.2, Aliouat-Denis C. M.2, Gay Melanie1 | ||||||||
Affiliation(s) | 1 : ANSES, Lab Securite Aliments, Dept Prod Peche & Aquaculture, Boulogne Sur Mer, France. 2 : Univ Lille Nord France, Inst Pasteur Lille, Ctr Infect & Immunite Lille, UMR CNRS 8204,Inserm U1019, F-59019 Lille, France. 3 : Genes Diffus, Douai, France. 4 : IFREMER, Nantes, France. 5 : Inst Pasteur, PEGASE Biosci, Lille, France. |
||||||||
Source | Journal Of Microbiological Methods (0167-7012) (Elsevier Science Bv), 2014-07 , Vol. 102 , P. 69-72 | ||||||||
DOI | 10.1016/j.mimet.2014.05.004 | ||||||||
WOS© Times Cited | 6 | ||||||||
Keyword(s) | Anisakidae, High-throughput sequencing, Nematode, Pooled DNA extraction, Marine fish, COX2 | ||||||||
Abstract | Different methods were evaluated to extract DNA from pooled nematodes belonging to Anisakis, Contracaecum, Pseudoterranova and Hysterothylacium genera isolated from edible fish. Pooled DNA extraction is the first and compulsory step to allow the identification of a large number of samples through high-throughput DNA sequencing with drastic time and cost reductions. | ||||||||
Full Text |
|