Monitoring of four DNA extraction methods upstream of high-throughput sequencing of Anisakidae nematodes

Type Article
Date 2014-07
Language English
Author(s) Seesao Y.1, 2, Audebert C.3, 5, Verrez-Bagnis VeroniqueORCID4, Merlin S.3, 5, Jerome Marc4, Viscogliosi E.2, Dei-Cas E.2, Aliouat-Denis C. M.2, Gay Melanie1
Affiliation(s) 1 : ANSES, Lab Securite Aliments, Dept Prod Peche & Aquaculture, Boulogne Sur Mer, France.
2 : Univ Lille Nord France, Inst Pasteur Lille, Ctr Infect & Immunite Lille, UMR CNRS 8204,Inserm U1019, F-59019 Lille, France.
3 : Genes Diffus, Douai, France.
4 : IFREMER, Nantes, France.
5 : Inst Pasteur, PEGASE Biosci, Lille, France.
Source Journal Of Microbiological Methods (0167-7012) (Elsevier Science Bv), 2014-07 , Vol. 102 , P. 69-72
DOI 10.1016/j.mimet.2014.05.004
WOS© Times Cited 6
Keyword(s) Anisakidae, High-throughput sequencing, Nematode, Pooled DNA extraction, Marine fish, COX2
Abstract Different methods were evaluated to extract DNA from pooled nematodes belonging to Anisakis, Contracaecum, Pseudoterranova and Hysterothylacium genera isolated from edible fish. Pooled DNA extraction is the first and compulsory step to allow the identification of a large number of samples through high-throughput DNA sequencing with drastic time and cost reductions.
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Seesao Y., Audebert C., Verrez-Bagnis Veronique, Merlin S., Jerome Marc, Viscogliosi E., Dei-Cas E., Aliouat-Denis C. M., Gay Melanie (2014). Monitoring of four DNA extraction methods upstream of high-throughput sequencing of Anisakidae nematodes. Journal Of Microbiological Methods, 102, 69-72. Publisher's official version : https://doi.org/10.1016/j.mimet.2014.05.004 , Open Access version : https://archimer.ifremer.fr/doc/00189/30020/