Monoclonal antibody against a gill Rickettsiales-like organism of Pecten maximus (Bivalvia): application to indirect immunofluorescence diagnosis

Type Article
Date 1992-12
Language English
Author(s) Le Gall Guillaume1, Mourton Chantal2, Boulo VivianeORCID1, Paolucci Francis3, Pau B2, Mialhe Eric1
Affiliation(s) 1 : IFREMER,LABEIM,UR PATHOL IMMUNOL BIOL MOLEC,BP 133,F-17390 LA TREMBLADE,FRANCE.
2 : FAC PHARM MONTPELLIER,F-34060 MONTPELLIER,FRANCE.
3 : SANOFI RECH,F-34082 MONTPELLIER,FRANCE.
Source Diseases Of Aquatic Organisms (0177-5103) (Inter-research), 1992-12 , Vol. 14 , N. 3 , P. 213-217
DOI 10.3354/dao014213
WOS© Times Cited 6
Abstract Using hybridoma technology, monoclonal antibodies (MABs) were prepared against a Rickettsiales-like organism (RLO) from the gills of the Saint-Jacques scallop Pecten maximus. The 5D7 MAB was then selected as a specific reagent suitable for an indirect immunofluorescence assay (IIFA) and evaluated for RLO diagnosis on P. maximus gill smears. Monoclonal antibody 5D7 showed no cross-reactivity with an RLO associated with a related pectinid species Chlamys opercularis.
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Le Gall Guillaume, Mourton Chantal, Boulo Viviane, Paolucci Francis, Pau B, Mialhe Eric (1992). Monoclonal antibody against a gill Rickettsiales-like organism of Pecten maximus (Bivalvia): application to indirect immunofluorescence diagnosis. Diseases Of Aquatic Organisms, 14(3), 213-217. Publisher's official version : https://doi.org/10.3354/dao014213 , Open Access version : https://archimer.ifremer.fr/doc/00190/30135/