We study the signature of rainfall on S1cm, the sea surface salinity retrieved from the Soil Moisture and Ocean Salinity (SMOS) satellite mission first by comparing SMOS S1cm with ARGO sea surface salinity measured at about 5 m depth in the Intertropical Convergence Zone (ITCZ) and in the Southern Pacific Convergence Zone; second by investigating spatial variability of SMOS S1cm related to rainfall. The resulting estimated S1cm decrease associated with rainfall occurring within less than 1 h from the salinity measurement is close to −0.2 pss (mm h−1) −1. We estimate that rain induced roughness and atmospheric effects are responsible for no more than 20% of this value. We also study the signature of rainfall on sea surface salinity measured by surface drifters at 45 cm depth and find a decrease associated with rainfall of −0.21 (±0.14) pss (mm h−1) −1, consistent with SMOS observations. When averaged over one month, this rain associated salinity decrease is at most −0.2 in monthly 100 × 100 km2 pixels, and at most 40% of the difference between SMOS S1cm and interpolated in situ bulk salinity in pixels near the ITCZ. This suggests that more than half of this difference is related to the in situ products obtained from optimal interpolation and therefore influenced by smoothing and relaxation to climatology. Finally, further studies on the satellite-derived salinities should pay attention to that as well as to other sources of uncertainties in satellite measurements and not interpret fully the observed differences between in situ and satellite mapped products, as rain induced SSS variability.