Pathogenic threats and probiotic use in larviculture of the scallop, Pecten maximus

Type Article
Date 2016-04
Language English
Author(s) Kesarcodi-Watson Aditya1, 2, Miner Philippe1, Nicolas Jean-Louis1, Asmani Katia1, Robert ReneORCID3
Affiliation(s) 1 : IFREMER Ctr Brest, Lab Physiol Invertebres, Plouzane, France.
2 : Cawthron Inst, Aquaculture & Biotechnol Dept, Nelson, New Zealand.
3 : IFREMER Ctr Brest, Stn Expt Argenton, Plouzane, France.
Source Aquaculture Research (1355-557X) (Wiley-blackwell), 2016-04 , Vol. 47 , N. 4 , P. 1221-1230
DOI 10.1111/are.12579
WOS© Times Cited 7
Keyword(s) scallop, Pecten maximus, probiotics, Alteromonas macleodii, Neptunomonas, Phaeobacter
Abstract During a series of experiments, bacteriological elements in scallop larval rearing were investigated: larvae susceptibility to pathogens as a function of their age, and the use of probiotic bacteria during larviculture. Younger larvae (d5 PF) were highly more susceptible to pathogenic-challenge than their older siblings, which were challenged at an older age (d15 PF). A challenge with 104 CFU mL−1 of V. pectenicida killed 100% of d5 PF larvae 7 days following challenge, yet killed only 9% of d15 PF larvae 9 days following challenge. Use of the probiotics Phaeobacter gallaeciensis, Alteromonas macleodii 0444 and Neptunomonas sp. 0536, provided for larger larvae, a high yield of competent larvae and, perhaps more importantly, protection against pathogen-challenge similar to levels achieved from antibiotic use. When challenged with V. pectenicida, d29 survivals were 20.3%, 85.1% and 75.0% respectively for control (no probiotic), antibiotic treated, and ‘probiotic mix’ administered larvae. Use of potential probiotic Pseudoalteromonas sp. D41 appeared to hinder scallop larvae. Future use of probiotics in scallop larval rearing would benefit from combined use of P. gallaeciensis, A. macleodii 0444 and Neptunomonas sp. 0536.
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