Methanococcoides vulcani sp nov., a marine methylotrophic methanogen that uses betaine, choline and N,N-dimethylethanolamine for methanogenesis, isolated from a mud volcano, and emended description of the genus Methanococcoides

Type Article
Date 2014-06
Language English
Author(s) L Haridon Stephane1, 2, 3, Chalopin MorganeORCID1, 2, 3, Colombo Delphine1, 2, 3, Toffin LaurentORCID1, 2, 3
Affiliation(s) 1 : UBO, UEB, Inst Univ Europeen Mer IUEM UMR 6197, LMEE, F-29280 Plouzane, France.
2 : CNRS, IUEM UMR 6197, LMEE, F-29280 Plouzane, France.
3 : IFREMER, UMR 6197, LMEE, F-29280 Plouzane, France.
Source International Journal Of Systematic And Evolutionary Microbiology (1466-5026) (Soc General Microbiology), 2014-06 , Vol. 64 , N. 6 , P. 1978-1983
DOI 10.1099/ijs.0.058289-0
WOS© Times Cited 23
Abstract A novel, strictly anaerobic, methylotrophic marine methanogen, strain SLH33(T), was isolated from deep sediment samples covered by an orange microbial mat collected from the Napoli Mud Volcano. Cells of strain SLH33(T) were Gram-stain-negative, motile, irregular cocci that occurred singly. Cells utilized trimethylamine, dimethylamine, monomethylamine, methanol, betaine, N,N-dimethylethanolamine and choline (N,N,N-trimethylethanolamine) as substrates for growth and methanogenesis. The optimal growth temperature was 30 degrees C; maximum growth rate was obtained at pH 7.0 in the presence of 0.5 M Na+. The DNA G+C content of strain SLH33(T) was 43.4 mol%. Phylogenetic analyses based on 16S rRNA gene sequences placed strain SLH33(T) within the genus Methanococcoides. The novel isolate was related most closely to Methanococcoides methylutens TMA-10(T) (98.8% 16S rRNA gene sequence similarity) but distantly related to Methanococcoides burtonii DSM 6242(T) (97.6%) and Methanococcoides alaskense AK-5(T) (97.6%). DNA DNA hybridization studies indicated that strain SLH33(T) represents a novel species, given that it shared less than 16% DNA DNA relatedness with Methanococcoides methylutens TMA-10(T). The name Methanococcoides vulcani sp. nov. is proposed for this novel species, with strain SLH33(T) (=DSM 26966(T)=JCM 19278(T)) as the type strain. An emended description of the genus Methanococcoides is also proposed.
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L Haridon Stephane, Chalopin Morgane, Colombo Delphine, Toffin Laurent (2014). Methanococcoides vulcani sp nov., a marine methylotrophic methanogen that uses betaine, choline and N,N-dimethylethanolamine for methanogenesis, isolated from a mud volcano, and emended description of the genus Methanococcoides. International Journal Of Systematic And Evolutionary Microbiology, 64(6), 1978-1983. Publisher's official version : https://doi.org/10.1099/ijs.0.058289-0 , Open Access version : https://archimer.ifremer.fr/doc/00211/32181/