The Medio-Basal Hypothalamus as a Dynamic and Plastic Reproduction-Related Kisspeptin-gnrh-Pituitary Center in Fish

Type Article
Date 2014-05
Language English
Author(s) Zmora Nilli1, Stubblefield John1, Golan Matan3, Servili Arianna4, Levavi-Sivan Berta3, Zohar Yonathan1, 2
Affiliation(s) 1 : Univ Maryland Baltimore Cty, Dept Marine Biotechnol, Baltimore, MD 21202 USA.
2 : Inst Marine & Environm Technol, Baltimore, MD 21202 USA.
3 : Hebrew Univ Jerusalem, Fac Agr Food & Environm, IL-76100 Rehobot, Israel.
4 : IFREMER, Unite Physiol Fonct Organismes Marins, Lab Sci Environm Marin, Unite Mixte Rech 6539, F-29280 Plouzane, France.
Source Endocrinology (0013-7227) (Endocrine Soc), 2014-05 , Vol. 155 , N. 5 , P. 1874-1886
DOI 10.1210/en.2013-1894
WOS© Times Cited 34
Abstract Kisspeptin regulates reproductive events, including puberty and ovulation, primarily via GnRH neurons. Prolonged treatment of prepubertal striped bass females with kisspeptin (Kiss) 1 or Kiss2 peptides failed to enhance puberty but suggested a gnrh-independent pituitary control pathway. Kiss2 inhibited, but Kiss1 stimulated, FSh beta expression and gonadal development, although hypophysiotropic gnrh1 and gnrh receptor expression remained unchanged. In situ hybridization and immunohistochemistry on brains and pituitaries revealed a differential plasticity between the 2 kisspeptin neurons. The differences were most pronounced at the prespawning phase in 2 regions along the path of gnrh1 axons: the nucleus lateralis tuberis (NLT) and the neurohypophysis. Kiss1 neurons appeared in the NLT and innervated the neurohypophysis of prespawning males and females, reaching Lh gonadotropes in the proximal pars distalis. Males, at all reproductive stages, had Kiss2 innervations in the NLT and the neurohypophysis, forming large axonal bundles in the former and intermingling with gnrh1 axons. Unlike in males, only preovulatory females had massive NLT-neurohypophysis staining of kiss2. Kiss2 neurons showed a distinct appearance in the NLT pars ventralis-equivalent region only in spawning zebrafish, indicating that this phenomenon is widespread. These results underscore the NLT as important nuclei for kisspeptin action in 2 facets: 1) kisspeptin-gnrh interaction, both kisspeptins are involved in the regulation of gnrh release, in a stage-and sex-dependent manner, especially at the prespawning phase; and 2) gnrh-independent effect of Kiss peptides on the pituitary, which together with the plastic nature of their neuronal projections to the pituitary implies that a direct gonadotropic regulation is plausible.
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Zmora Nilli, Stubblefield John, Golan Matan, Servili Arianna, Levavi-Sivan Berta, Zohar Yonathan (2014). The Medio-Basal Hypothalamus as a Dynamic and Plastic Reproduction-Related Kisspeptin-gnrh-Pituitary Center in Fish. Endocrinology, 155(5), 1874-1886. Publisher's official version : https://doi.org/10.1210/en.2013-1894 , Open Access version : https://archimer.ifremer.fr/doc/00217/32873/