High or low dietary carbohydrate: protein ratios during first-feeding affect glucose metabolism and intestinal microbiota in juvenile rainbow trout

Type Article
Date 2014-10
Language English
Author(s) Geurden I.1, Mennigen J.1, Plagnes-Juan E.1, Veron V.1, Cerezo T.1, Mazurais DavidORCID2, Zambonino-Infante Jose-LuisORCID2, Gatesoupe Joel1, 2, Skiba-Cassy S.1, Panserat S.1
Affiliation(s) 1 : INRA, Nutr Metab & Aquaculture UR1067, F-64310 St Pee Sur Nivelle, France.
2 : IFREMER, UMR LEMAR 6539, Lab Adaptat Reprod & Nutr Poissons ARN, Lab Sci Environm Marin, F-29280 Plouzane, France.
Source Journal Of Experimental Biology (0022-0949) (Company Of Biologists Ltd), 2014-10 , Vol. 217 , N. 19 , P. 3396-3406
DOI 10.1242/jeb.106062
WOS© Times Cited 59
Keyword(s) Nutritional programming, Rainbow trout, Carbohydrates, Protein, Metabolism
Abstract Based on the concept of nutritional programming in mammals, we tested whether an acute hyperglucidic-hypoproteic stimulus during first feeding could induce long-term changes in nutrient metabolism in rainbow trout. Trout alevins received during the five first days of exogenous feeding either a hyperglucidic (40% gelatinized starch + 20% glucose) and hypoproteic (20%) diet (VLP diet) or a high-protein (60%) glucose-free diet (HP diet, control). Following a common 105-day period on a commercial diet, both groups were then challenged (65 days) with a carbohydrate-rich diet (28%). Short-and long-term effects of the early stimuli were evaluated in terms of metabolic marker gene expressions and intestinal microbiota as initial gut colonisation is essential for regulating the development of the digestive system. In whole alevins (short term), diet VLP relative to HP rapidly increased gene expressions of glycolytic enzymes, while those involved in gluconeogenesis and amino acid catabolism decreased. However, none of these genes showed persistent molecular adaptation in the liver of challenged juveniles (long term). By contrast, muscle of challenged juveniles subjected previously to the VLP stimulus displayed downregulated expression of markers of glycolysis and glucose transport (not seen in the short term). These fish also had higher plasma glucose (9 h postprandial), suggesting impaired glucose homeostasis induced by the early stimulus. The early stimulus did not modify the expression of the analysed metabolism-related microRNAs, but had short-and long-term effects on intestinal fungi (not bacteria) profiles. In summary, our data show that a short hyperglucidic-hypoproteic stimulus during early life may have a long-term influence on muscle glucose metabolism and intestinal microbiota in trout.
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Geurden I., Mennigen J., Plagnes-Juan E., Veron V., Cerezo T., Mazurais David, Zambonino-Infante Jose-Luis, Gatesoupe Joel, Skiba-Cassy S., Panserat S. (2014). High or low dietary carbohydrate: protein ratios during first-feeding affect glucose metabolism and intestinal microbiota in juvenile rainbow trout. Journal Of Experimental Biology, 217(19), 3396-3406. Publisher's official version : https://doi.org/10.1242/jeb.106062 , Open Access version : https://archimer.ifremer.fr/doc/00221/33264/