Genetic diversity of the harmful family Kareniaceae (Gymnodiniales, Dinophyceae) in France, with the description of Karlodinium gentienii sp. nov.: A new potentially toxic dinoflagellate

Type Publication
Date 2014-12
Language English
Copyright 2014 Elsevier B.V. All rights reserved.
Author(s) Nezan Elisabeth1, Siano Raffaele2, Boulben Sylviane1, Six Christophe3, 4, Bilien GwenaelORCID1, Cheze Karine5, Duval Audrey1, Le Panse Sophie6, Quere Julien2, Chomerat Nicolas1
Affiliation(s) 1 : IFREMER, Station de Biologie Marine, Place de la Croix, BP 40537, F-29185 Concarneau Cedex, France
2 : IFREMER, Centre de Bretagne, DYNECO/Pelagos, BP 70, F-29280 Plouzané, France
3 : Sorbonne Universities, Pierre et Marie Curie University (Paris 06), UMR 7144, Marine Phototrophic Prokaryotes Group, Station Biologique de Roscoff, 29688 Roscoff Cedex, France
4 : Centre National pour la Recherche Scientifique, UMR 7144, Marine Phototrophic Prokaryotes Group, Station Biologique de Roscoff, Place Georges Teissier, CS90074, 29688 Roscoff Cedex, France
5 : Muséum National d’Histoire Naturelle, Station de Biologie Marine, BP 225, F-29182 Concarneau Cedex, France
6 : Centre National pour la Recherche Scientifique, Fr 2424, Plate-forme Merimagerie, Station Biologique de Roscoff, Place Georges Teissier, CS90074, 29688 Roscoff Cedex, France
Source Harmful Algae (1568-9883) (Elsevier), 2014-12 , Vol. 40 , P. 75-91
DOI 10.1016/j.hal.2014.10.006
WOS© Times Cited 15
Keyword(s) Genetic diversity, Haemolytic, Kareniaceae, Pigments, Single cell, Taxonomy
Abstract The family Kareniaceae is mostly known in France for recurrent blooms of Karenia mikimotoi in the Atlantic, English Channel, and Mediterranean Sea and for the unusual green discoloration in the saltwater lagoon of Diana (Corsica) caused by Karlodinium corsicum in April 1994. In terms of diversity, this taxonomic group was long overlooked owing to the difficult identification of these small unarmored dinoflagellates. In this study, thanks to the molecular characterization performed on single cells from field samples and cultures, twelve taxonomic units were assigned to the known genera Karenia, Karlodinium and Takayama, whereas one could not be affiliated to any described genus. The molecular phylogeny inferred from the D1–D2 region of the LSU rDNA showed that five of them formed a sister taxon of a known species, and could not be identified at species-level, on the basis of molecular analysis only. Among these latter taxa, one Karlodinium which was successfully cultured was investigated by studying the external morphological features (using two procedures for cells fixation), ultrastructure, pigment composition, and haemolytic activity. The results of our analyses corroborate the genetic results in favour of the erection of Karlodinium gentienii sp. nov., which possesses an internal complex system of trichocysts connected to external micro-processes particularly abundant in the epicone, and a peculiar pigment composition. In addition, preliminary assays showed a haemolytic activity.
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Nezan Elisabeth, Siano Raffaele, Boulben Sylviane, Six Christophe, Bilien Gwenael, Cheze Karine, Duval Audrey, Le Panse Sophie, Quere Julien, Chomerat Nicolas (2014). Genetic diversity of the harmful family Kareniaceae (Gymnodiniales, Dinophyceae) in France, with the description of Karlodinium gentienii sp. nov.: A new potentially toxic dinoflagellate. Harmful Algae, 40, 75-91. Publisher's official version : https://doi.org/10.1016/j.hal.2014.10.006 , Open Access version : https://archimer.ifremer.fr/doc/00225/33611/