A single regulatory gene is sufficient to alter Vibrio aestuarianus pathogenicity in oysters

Type Article
Date 2015-11
Language English
Author(s) Goudenege David1, 2, 3, Travers Marie-AgnesORCID4, Lemire Astrid1, 2, 3, Petton Bruno1, Haffner Philippe4, Labreuche Yannick1, 2, 3, Tourbiez Delphine4, Mangenot Sophie5, Calteau Alexandra5, 6, Mazel Didier7, 8, Nicolas Jean-Louis1, Jacq Annick9, Le Roux Frederique1, 2, 3
Affiliation(s) 1 : IFREMER, Unite Physiol Fonct Organismes Marins, F-29280 Plouzane, France.
2 : UPMC Univ Paris, Univ Paris 04, Stn Biol Roscoff, Integrat Biol Marine Models,UMR 8227, F-29688 Roscoff, France.
3 : CNRS, Integrat Biol Marine Models, UMR 8227, F-29688 Roscoff, France.
4 : IFREMER, Lab Genet & Pathol Mollusques Marins Ave Mus Loup, F-17390 La Tremblade, France.
5 : Commissariat Energie Atom & Energies Alternat CE, IG, DSV, F-91057 Evry, France.
6 : CNRS, LABGeM, UMR 8030, X-91057 Evry, France.
7 : Inst Pasteur, Dept Genomes & Genet, Unite Plasticite Genome Bacterien, F-75015 Paris, France.
8 : CNRS, UMR 3525, F-75015 Paris, France.
9 : Univ Paris 11, CNRS, Inst Genet & Microbiol, UMR 8621, F-91405 Orsay, France.
Source Environmental Microbiology (1462-2912) (Wiley-blackwell), 2015-11 , Vol. 17 , N. 11 , P. 4189-4199
DOI 10.1111/1462-2920.12699
WOS© Times Cited 21
Note Special Issue: Pathogen Ecology
Abstract Oyster diseases caused by pathogenic vibrios pose a major challenge to the sustainability of oyster farming. In France since 2012 a disease affecting specifically adult oysters has been associated with the presence of Vibrio aestuarianus. Here, by combining genome comparison, phylogenetic analyses and high throughput infections of strains isolated before or during the recent outbreaks, we show that virulent strains cluster into two V. aestuarianus lineages independently of the sampling dates. The bacterial lethal dose was not different between strains isolated before or after 2012. Hence the emergence of a new highly virulent clonal strain is unlikely. Each lineage comprises nearly identical strains, the majority of them being virulent, suggesting that within these phylogenetically coherent virulent lineages a few strains has lost their pathogenicity. Comparative genomics allowed the identification of a single frameshift in a non-virulent strain. This mutation affects the varS gene that codes for a signal transduction histidine-protein kinase. Genetic analyses confirmed that varS is necessary for infection of oysters and for a secreted metalloprotease expression. For the first time in a Vibrio species we show here that VarS is a key factor of pathogenicity
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Goudenege David, Travers Marie-Agnes, Lemire Astrid, Petton Bruno, Haffner Philippe, Labreuche Yannick, Tourbiez Delphine, Mangenot Sophie, Calteau Alexandra, Mazel Didier, Nicolas Jean-Louis, Jacq Annick, Le Roux Frederique (2015). A single regulatory gene is sufficient to alter Vibrio aestuarianus pathogenicity in oysters. Environmental Microbiology, 17(11), 4189-4199. Publisher's official version : https://doi.org/10.1111/1462-2920.12699 , Open Access version : https://archimer.ifremer.fr/doc/00226/33685/