Genome sequence of Vibrio diabolicus and identification of the exopolysaccharide HE800 biosynthesis locus

Type Article
Date 2014-12
Language English
Author(s) Goudenege David2, 3, 4, Boursicot Vincent1, Versigny Typhaine1, Bonnetot Sandrine1, Ratiskol Jacqueline1, Sinquin CorinneORCID1, Lapointe Gisele5, Le Roux Frederique2, 3, 4, Delbarre-Ladrat ChristineORCID1
Affiliation(s) 1 : Ifremer, Laboratoire EM3B (Ecosystèmes Marins et Molécules Marines pour les Biotechnologies), Rue de l’Ile d’Yeu, BP21105, F-44311, Nantes Cedex 3, France
2 : Ifremer, Unité Physiologie Fonctionnelle des Organismes Marins, ZI de la Pointe du Diable, CS 10070, F-29280, Plouzané, France
3 : Sorbonne Universités, UPMC Univ Paris 06, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074, F-29688, Roscoff Cedex, France
4 : CNRS, UMR 8227, Integrative Biology of Marine Models, Station Biologique de Roscoff, CS 90074, F-29688, Roscoff Cedex, France
5 : Université Laval, Institute for Nutrition and Functional Foods, 2440 boul. Hochelaga, Québec, Québec, Canada
Source Applied Microbiology And Biotechnology (1432-0614), 2014-12 , Vol. 98 , N. 24 , P. 10165–1017
DOI 10.1007/s00253-014-6086-8
WOS© Times Cited 10
Keyword(s) Genome, Vibrio, Exopolysaccharide, Biosynthesis
Abstract Vibrio diabolicus, a marine bacterium originating from deep-sea hydrothermal vents, produces the HE800 exopolysaccharide with high value for biotechnological purposes, especially for human health. Its genome was sequenced and analyzed; phylogenetic analysis using the core genome revealed V. diabolicus is close to another deep-sea Vibrio sp. (Ex25) within the Harveyi clade and Alginolyticus group. A genetic locus homologous to the syp cluster from Vibrio fischeri was demonstrated to be involved in the HE800 production. However, few genetic particularities suggest that the regulation of syp expression may be different in V. diabolicus. The presence of several types of glycosyltransferases within the locus indicates a capacity to generate diversity in the glycosidic structure, which may confer an adaptability to environmental conditions. These results contribute to better understanding exopolysaccharide biosynthesis and for developing new efficient processes to produce this molecule for biotechnological applications.
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Goudenege David, Boursicot Vincent, Versigny Typhaine, Bonnetot Sandrine, Ratiskol Jacqueline, Sinquin Corinne, Lapointe Gisele, Le Roux Frederique, Delbarre-Ladrat Christine (2014). Genome sequence of Vibrio diabolicus and identification of the exopolysaccharide HE800 biosynthesis locus. Applied Microbiology And Biotechnology, 98(24), 10165–1017. Publisher's official version : https://doi.org/10.1007/s00253-014-6086-8 , Open Access version : https://archimer.ifremer.fr/doc/00241/35244/