Thermosipho activus sp nov., a thermophilic, anaerobic, hydrolytic bacterium isolated from a deep-sea sample
| Type | Article | ||||||||
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| Date | 2014-09 | ||||||||
| Language | English | ||||||||
| Author(s) | Podosokorskaya Olga A.1, Bonch-Osmolovskaya Elizaveta A.1, Godfroy Anne 2, Gavrilov Sergey N.1, Beskorovaynaya Daria A.3, Sokolova Tatyana G.1, Kolganova Tatyana V.4, Toshchakov Stepan V.5, Kublanov Ilya V.1 |
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| Affiliation(s) | 1 : Winogradsky Inst Microbiol, Russian Acad Sci, Moscow 117312, Russia. 2 : IFREMER, Lab Microbiol Environm Extremes, Univ Brest, CNRS,UMR6197,Unite Etud Ecosyst Profonds, F-29280 Plouzane, France. 3 : Gubkin Russian State Univ Oil & Gas, Moscow 119991, Russia. 4 : Bioengn Ctr, Russian Acad Sci, Moscow 117312, Russia. 5 : Immanuel Kant Balt Fed Univ, Kaliningrad 236016, Russia. |
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| Source | International Journal Of Systematic And Evolutionary Microbiology (1466-5026) (Soc General Microbiology), 2014-09 , Vol. 64 , P. 3307-3313 | ||||||||
| DOI | 10.1099/ijs.0.063156-0 | ||||||||
| WOS© Times Cited | 10 | ||||||||
| Abstract | A novel obligately anaerobic, extremely thermophilic, organotrophic bacterium, strain Rift-s3(T), was isolated from a deep-sea sample containing Riftia pachyptila sheath from Guaymas Basin, Gulf of California. Cells of the novel isolate were rods, 0.3-0.8 mu m in width and 1.5-10 mu m in length, surrounded by a sheath-like structure (toga). Strain Rift-s3(T) grew at temperatures ranging from 44 to 75 degrees C, at pH 5.5 to 8.0, and with NaCI concentrations of 3 to 60 g l(-1). Under optimum conditions (65 degrees C, pH 6.0, NaCl 25 g I-1), the doubling time was 30 min. The isolate was able to ferment mono-, oligo- and polysaccharides including cellulose, chitin, xylan and: pectin, and proteins including beta-keratins, casein and gelatin. Acetate, hydrogen and carbon dioxide were the main products of glucose fermentation. The G +C content of the DNA was 30 mol%. Phylogenetic analysis of 16S rRNA gene sequences showed the affiliation of strain Rift-s3(T) with the genus Thermosipho, with Thermosipho atlanticus Ob7(T) as the closest relative (96.5% 16S rRNA gene sequence similarity). Based on the phylogenetic analysis and physiological properties of the novel isolate we propose a novel species of the genus Thermosipho, Thermosipho activus sp. nov., with Rift-s3(T) (=DSM 26467(T)=VKM B-2803(T)) as the type strain. | ||||||||
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