High spatial variability of phytoplankton assessed by flow cytometry, in a dynamic productive coastal area, in spring:the eastern English Channel

Type Article
Date 2015-03
Language English
Author(s) Bonato Simon1, Christaki Urania1, Lefebvre AlainORCID2, Lizon Fabrice3, Thyssen Melilotus4, Artigas Luis Felipe1
Affiliation(s) 1 : Univ Littoral Cote dOpale, Lab Oceanol & Geosci, CNRS UMR LOG 8187, MREN ULCO, F-62930 Wimereux, France.
2 : Ifremer LER Boulogne, Lab Environm Littoral & Ressoumes Aquacoles, F-62321 Boulogne Sur Mer, France.
3 : Univ Sci & Technol Lille Lille 1, Lab Oceanol & Geosci, CNRS UMR LOG 8187, Stn Marine Wimereux, F-62930 Wimereux, France.
4 : Aix Marseille Univ, MIO, UM110, CNRS INSU IRD, F-13288 Marseille 09, France.
Source Estuarine Coastal And Shelf Science (0272-7714) (Academic Press Ltd- Elsevier Science Ltd), 2015-03 , Vol. 154 , P. 214-223
DOI 10.1016/j.ecss.2014.12.037
WOS© Times Cited 23
Keyword(s) phytoplankton dynamics, high spatial resolution, flow cytometer, red fluorescence
Abstract The distribution of phytoplankton (from pico-to microphytoplankton) was investigated, at single-cell level and at high spatial resolution, during an oceanographic cruise across the eastern English Channel (EEC) between April 27 and 29, 2012. Seawater was continuously collected from surface waters and analysed on board at high frequency (one sample every 10 min), by using a new generation of pulse-shape recording scanning flow cytometer (CytoSense, Cytobuoy©). A Bray-Curtis matrix analysis based on phytoplankton composition allowed the discrimination of 4 communities. Within these communities, abundance, cell size as well as single cell and total red fluorescence of 8 phytoplankton groups were measured. Picoeukaryotes and Synechococcus spp. cells dominated the mid Channel and most of the English waters monitored, whereas waters off Eastbourne as well as French coastal waters (under remote and direct estuarine influence) were characterized by the dominance of Phaeocystis globosa haploid and diploid cells. Most of the total red fluorescence signal, which correlated with chlorophyll a concentrations, was attributable to P. globosa and, to a lesser extent, to diatoms. In addition to sub-mesoscale variation within phytoplankton communities, the single-cell features within each phytoplankton group gave information about the physiological status of individual phytoplankton cells.
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Bonato Simon, Christaki Urania, Lefebvre Alain, Lizon Fabrice, Thyssen Melilotus, Artigas Luis Felipe (2015). High spatial variability of phytoplankton assessed by flow cytometry, in a dynamic productive coastal area, in spring:the eastern English Channel. Estuarine Coastal And Shelf Science, 154, 214-223. Publisher's official version : https://doi.org/10.1016/j.ecss.2014.12.037 , Open Access version : https://archimer.ifremer.fr/doc/00248/35885/