Influence of one selected Tisochrysis lutea strain rich in lipids on Crassostrea gigas larval development and biochemical composition
|Author(s)||Da Costa F.1, 2, Petton Bruno1, Mingant Christian1, Bougaran Gael3, Rouxel Catherine3, Quere Claudie1, Wikfors G. H.4, Soudant P.5, Robert Rene1, 6|
|Affiliation(s)||1 : IFREMER, Lab Sci Environm Marin, UMR 6539, LEMAR, Plouzane, France.
2 : Novostrea Bretagne, Sarzeau, France.
3 : IFREMER, Lab Physiol & Biotechnol Algues, Nantes 3, France.
4 : NOAA, Northeast Fisheries Sci Ctr, NMFS, Milford, CT USA.
5 : IUEM UBO, Lab Sci Environm Marin, LEMAR, UMR 6539, Plouzane, France.
6 : IFREMER, Unite Littoral, Ctr Bretagne ZI Pointe Diable, F-29280 Plouzane, France.
|Source||Aquaculture Nutrition (1353-5773) (Wiley-blackwell), 2016-08 , Vol. 22 , N. 4 , P. 813-836|
|WOS© Times Cited||16|
|Keyword(s)||larvae, lipids, oyster, Tisochrysis lutea|
|Abstract||Effects of a remarkably high overall lipid Tisochrysis lutea strain (T+) upon gross biochemical composition, fatty acid (FA), sterol and lipid class composition of Crassostrea gigas larvae were evaluated and compared with a normal strain of Tisochrysis lutea (T) and the diatom Chaetoceros neogracile (Cg). In a first experiment, the influence of different single diets (T, T+ and Cg) and a bispecific diet (TCg) was studied, whereas, effects of monospecific diets (T and T+) and bispecific diets (TCg and T+Cg) were evaluated in a second experiment. The strain T+ was very rich in triglycerides (TAG: 93–95% of total neutral lipids), saturated FA (45%), monounsaturated FA (31–33%) and total fatty acids (4.0–4.7 pg cell−1). Larval oyster survival and growth rate were positively correlated with 18:1n-7 and 20:1n-7, in storage lipids (SL), and negatively related to 14:0, 18:1n-9, 20:1n-9, 20:4n-6 and trans-22-dehydrocholesterol in membrane lipids (ML). Surprisingly, only the essential fatty acid 20:5n-3 in SL was correlated positively with larval survival. Correlations suggest that physiological disruption by overabundance of TAG, FFA and certain fatty acids in larvae fed T+ was largely responsible for the poor performance of these larvae. ‘High-lipid’ strains of microalgae, without regard to qualitative lipid composition, do not always improve bivalve larval performance.|