Influence of one selected Tisochrysis lutea strain rich in lipids on Crassostrea gigas larval development and biochemical composition

Type Article
Date 2016-08
Language English
Author(s) Da Costa F.1, 2, Petton Bruno1, Mingant Christian1, Bougaran GaelORCID3, Rouxel Catherine3, Quere Claudie1, Wikfors G. H.4, Soudant P.5, Robert ReneORCID1, 6
Affiliation(s) 1 : IFREMER, Lab Sci Environm Marin, UMR 6539, LEMAR, Plouzane, France.
2 : Novostrea Bretagne, Sarzeau, France.
3 : IFREMER, Lab Physiol & Biotechnol Algues, Nantes 3, France.
4 : NOAA, Northeast Fisheries Sci Ctr, NMFS, Milford, CT USA.
5 : IUEM UBO, Lab Sci Environm Marin, LEMAR, UMR 6539, Plouzane, France.
6 : IFREMER, Unite Littoral, Ctr Bretagne ZI Pointe Diable, F-29280 Plouzane, France.
Source Aquaculture Nutrition (1353-5773) (Wiley-blackwell), 2016-08 , Vol. 22 , N. 4 , P. 813-836
DOI 10.1111/anu.12301
WOS© Times Cited 7
Keyword(s) larvae, lipids, oyster, Tisochrysis lutea
Abstract Effects of a remarkably high overall lipid Tisochrysis lutea strain (T+) upon gross biochemical composition, fatty acid (FA), sterol and lipid class composition of Crassostrea gigas larvae were evaluated and compared with a normal strain of Tisochrysis lutea (T) and the diatom Chaetoceros neogracile (Cg). In a first experiment, the influence of different single diets (T, T+ and Cg) and a bispecific diet (TCg) was studied, whereas, effects of monospecific diets (T and T+) and bispecific diets (TCg and T+Cg) were evaluated in a second experiment. The strain T+ was very rich in triglycerides (TAG: 93–95% of total neutral lipids), saturated FA (45%), monounsaturated FA (31–33%) and total fatty acids (4.0–4.7 pg cell−1). Larval oyster survival and growth rate were positively correlated with 18:1n-7 and 20:1n-7, in storage lipids (SL), and negatively related to 14:0, 18:1n-9, 20:1n-9, 20:4n-6 and trans-22-dehydrocholesterol in membrane lipids (ML). Surprisingly, only the essential fatty acid 20:5n-3 in SL was correlated positively with larval survival. Correlations suggest that physiological disruption by overabundance of TAG, FFA and certain fatty acids in larvae fed T+ was largely responsible for the poor performance of these larvae. ‘High-lipid’ strains of microalgae, without regard to qualitative lipid composition, do not always improve bivalve larval performance.
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Da Costa F., Petton Bruno, Mingant Christian, Bougaran Gael, Rouxel Catherine, Quere Claudie, Wikfors G. H., Soudant P., Robert Rene (2016). Influence of one selected Tisochrysis lutea strain rich in lipids on Crassostrea gigas larval development and biochemical composition. Aquaculture Nutrition, 22(4), 813-836. Publisher's official version : https://doi.org/10.1111/anu.12301 , Open Access version : https://archimer.ifremer.fr/doc/00251/36258/