Factors other than metalloprotease are required for full virulence of French Vibrio tubiashii isolates in oyster larvae

Type Article
Date 2015-05
Language English
Author(s) Mersni-Achour Rachida1, 2, 3, Ben Cheikh Yosra1, Pichereau Vianney4, Doghri Ibtissem1, Etien Cedric1, Degremont LionelORCID2, Saulnier Denis2, 5, Fruitier-Arnaudin Ingrid1, 3, Travers Marie-AgnesORCID2
Affiliation(s) 1 : Univ La Rochelle, CNRS ULR, LIENSs, Equipe Approches Mol,Environm Sante,UMR 7266, F-17042 La Rochelle, France.
2 : IFREMER, SG2M LGPMM, Lab Genet & Pathol Mollusques Marins, F-17390 La Tremblade, France.
3 : Univ La Rochelle, Federat Rech Environm & Dev Durable, FR CNRS 3097, La Rochelle, France.
4 : Univ Europeenne Bretagne, Lab Sci Environnement Mann LEMAR, CNRS,IFREMER,UBO,IRD, Inst Univ Europeen Mer,Univ Bretagne Occidentale, F-29280 Plouzane, France.
5 : IFREMER, Ctr Iftemer Pacifique, UMR 241, Ecosysternes Lnsulaires Oceaniens, Tahiti 98719, Taravao, Fr Polynesia.
Source Microbiology-sgm (1350-0872) (Soc General Microbiology), 2015-05 , Vol. 161 , P. 997-1007
DOI 10.1099/mic.0.000058
WOS© Times Cited 7
Abstract Vibrio tubiashii is a marine pathogen isolated from larval and juvenile bivalve molluscs causing bacillary necrosis. Recent studies demonstrated the isolation of this species in a French experimental hatchery/nursery affecting Crassostrea gigas spat in 2007. Here, using larvae of C. gigas as an interaction model, we showed that the French V. tubiashii is virulent to larvae and can cause bacillary necrosis symptoms with a LD50 about 2.3 × 103 cfu ml-1 after 24 h. Moreover, complete or GP- HPLC fractionated extracellular products (ECPs) of this strain appeared toxic to larvae. MS-MS analysis of the different ECPs fractions revealed the existence of an extracellular metalloprotease and other suspected virulence factors. This observation is also supported by the expression level of some potential virulence factors. The
overall results suggest that the pathology caused by the French V. tubiashii in C. gigas oysters is caused by a group of toxic factors and not only the metalloprotease
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Mersni-Achour Rachida, Ben Cheikh Yosra, Pichereau Vianney, Doghri Ibtissem, Etien Cedric, Degremont Lionel, Saulnier Denis, Fruitier-Arnaudin Ingrid, Travers Marie-Agnes (2015). Factors other than metalloprotease are required for full virulence of French Vibrio tubiashii isolates in oyster larvae. Microbiology-sgm, 161, 997-1007. Publisher's official version : https://doi.org/10.1099/mic.0.000058 , Open Access version : https://archimer.ifremer.fr/doc/00252/36367/