The marine bacteria Cobetia marina DSMZ 4741 synthesizes an unexpected K-antigen-like exopolysaccharide

Type Article
Date 2015-06
Language English
Author(s) Lelchat Florian1, Cerantola Stephane2, Brandily Christophe1, Colliec-Jouault SylviaORCID1, Baudoux Anne-Claire3, 4, Ojima Takao5, Boisset Claire6
Affiliation(s) 1 : Ctr Ifremer Brest, Lab BMM, F-29280 Plouzane, France.
2 : Univ Bretagne Occidentale, Fac Sci Brest, Serv Commun Resonnance Magnet Nucl, F-29238 Brest 3, France.
3 : Univ Paris 06, Univ Paris 04, Stn Biol Roscoff, UMR 7144, F-29680 Roscoff, France.
4 : CNRS, Stn Biol Roscoff, UMR 7144, F-29680 Roscoff, France.
5 : Hokkaido Univ, Grad Sch Fisheries Sci, Lab Marine Biotechnol & Microbiol, Hakodate, Hokkaido 0418611, Japan.
6 : CERMAV CNRS, Serv Commun Chromatog, F-38041 Grenoble, France.
Source Carbohydrate Polymers (0144-8617) (Elsevier Sci Ltd), 2015-06 , Vol. 124 , P. 347-356
DOI 10.1016/j.carbpol.2015.02.038
WOS© Times Cited 14
Keyword(s) Bacterial exopolysaccharides, K-antigen, Cobetia marina, KDO, Marine polysaccharide
Abstract We have studied the exopolysaccharide produced by Cobetia marina DSMZ 4741, a marine bacterium isolated from coastal seawater. This strain is able to produce a polysaccharide in presence of carbon sources as glucose, mannitol and alginate. The maximum production occurs in aerobic condition, during the end of the exponential phase. The polymer is a non-viscous, acidic heteropolysaccharide of 270 kDa constituted of a repeating unit of: →2)-β-D-Ribf-(1→4)-[7,8-O-(Pyr)]-α-D-KDOp-(2→ This kind of chemical structure is generally related to K-antigen polysaccharide of pathogenic Escherichia coli strains. This is the first time this type of EPS is described from a marine bacterium. Moreover the polysaccharide exhibits a pyruvate substitution on its 3-deoxy-D-manno-oct-2-ulosonic acid (KDO) residue never encountered before. The discovery of such an unexpected EPS with high biotechnological potential is a new incentive for a better exploration of bioactive marine resources.
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