Evidence of active methanogen communities in shallow sediments of the Sonora Margin cold seeps

Type Article
Date 2015-05
Language English
Author(s) Vigneron AdrienORCID1, 2, 3, 5, L Haridon Stephane2, 3, Godfroy AnneORCID1, 2, 3, Roussel ErwanORCID1, 2, 3, Cragg Barry A.4, Parkes R. John4, Toffin LaurentORCID1, 2, 3
Affiliation(s) 1 : IFREMER, Lab Microbiol Environm Extremes, Technopole Brest Iroise, UMR6197, Plouzane, France.
2 : Univ Bretagne Occidentale, Lab Microbiol Environm Extremes, Technopole Brest Iroise, UMR6197, Plouzane, France.
3 : CNRS, Lab Microbiol Environm Extremes, Technopole Brest Iroise, UMR6197, Plouzane, France.
4 : Cardiff Univ, Sch Earth & Ocean Sci, Cardiff CF10 3AX, S Glam, Wales.
5 : Newcastle Univ, Sch Civil Engn & Geosci, Newcastle Upon Tyne NE1 7RU, Tyne & Wear, England.
Source Applied And Environmental Microbiology (0099-2240) (Amer Soc Microbiology), 2015-05 , Vol. 81 , N. 10 , P. 3451-3459
DOI 10.1128/AEM.00147-15
WOS© Times Cited 12
Abstract In the Sonora Margin cold seep ecosystems (Gulf of California), sediments underlying microbial mats harbor high biogenic methane concentrations, fueling various microbial communities, such as abundant lineages of anaerobic methanotrophs (ANME). However, the biodiversity, distribution, and metabolism of the microorganisms producing this methane remain poorly understood. In this study, measurements of methanogenesis using radiolabeled dimethylamine, bicarbonate, and acetate showed that biogenic methane production in these sediments was mainly dominated by methylotrophic methanogenesis, while the proportion of autotrophic methanogenesis increased with depth. Congruently, methane production and methanogenic Archaea were detected in culture enrichments amended with trimethylamine and bicarbonate. Analyses of denaturing gradient gel electrophoresis (DGGE) fingerprinting and reverse-transcribed PCR-amplified 16S rRNA sequences retrieved from these enrichments revealed the presence of active methylotrophic Methanococcoides burtonii relatives and several new autotrophic Methanogenium lineages, confirming the cooccurrence of Methanosarcinales and Methanomicrobiales methanogens with abundant ANME populations in the sediments of the Sonora Margin cold seeps.
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Vigneron Adrien, L Haridon Stephane, Godfroy Anne, Roussel Erwan, Cragg Barry A., Parkes R. John, Toffin Laurent (2015). Evidence of active methanogen communities in shallow sediments of the Sonora Margin cold seeps. Applied And Environmental Microbiology, 81(10), 3451-3459. Publisher's official version : https://doi.org/10.1128/AEM.00147-15 , Open Access version : https://archimer.ifremer.fr/doc/00256/36737/