The use of fluorescent Nile red and BODIPY for lipid measurement in microalgae

Type Article
Date 2015-03
Language English
Author(s) Rumin Judith1, Bonnefond Hubert2, 4, Saint-Jean Bruno1, Rouxel Catherine1, Sciandra Antoine2, 4, Bernard Olivier3, Cadoret Jean-Paul1, Bougaran GaelORCID1
Affiliation(s) 1 : IFREMER, PBA, F-44311 Nantes, France.
2 : CNRS, UMPC, LOV UMR 7093, F-06230 Villefranche Sur Mer, France.
3 : INRIA BIOCORE, F-06902 Sophia Antipolis, France.
Source Biotechnology For Biofuels (1754-6834) (Biomed Central Ltd), 2015-03 , Vol. 8 , N. 42 , P. 1-16
DOI 10.1186/s13068-015-0220-4
WOS© Times Cited 75
Keyword(s) Nile red, BODIPY 505/515, Microalgae, Neutral lipid, Fluorescence, Biodiesel
Abstract Microalgae are currently emerging as one of the most promising alternative sources for the next generation of food, feed, cosmetics and renewable energy in the form of biofuel. Microalgae constitute a diverse group of microorganisms with advantages like fast and efficient growth. In addition, they do not compete for arable land and offer very high lipid yield potential. Major challenges for the development of this resource are to select lipid-rich strains using high-throughput staining for neutral lipid content in microalgae species. For this purpose, the fluorescent dyes most commonly used to quantify lipids are Nile red and BODIPY 505/515. Their fluorescent staining for lipids offers a rapid and inexpensive analysis tool to measure neutral lipid content, avoiding time-consuming and costly gravimetric analysis. This review collates and presents recent advances in algal lipid staining and focuses on Nile red and BODIPY 505/515 staining characteristics. The available literature addresses the limitations of fluorescent dyes under certain conditions, such as spectral properties, dye concentrations, cell concentrations, temperature and incubation duration. Moreover, the overall conclusion of the present review study gives limitations on the use of fluorochrome for screening of lipid-rich microalgae species and suggests improved protocols for staining recalcitrant microalgae and recommendations for the staining quantification.
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