Exposures of zebrafish through diet to three environmentally relevant mixtures of PAHs produce behavioral disruptions in unexposed F1 and F2 descendant

Type Article
Date 2015-11
Language English
Author(s) Vignet Caroline1, Joassard Lucette2, Lyphout Laura2, Guionnet Tiphaine2, Goubeau Manon1, Le Menach Karyn3, Brion Francois4, Kah Olivier5, Chung Bon-Chu6, Budzinski Helene3, Begout Marie-LaureORCID2, Cousin XavierORCID1, 7
Affiliation(s) 1 : IFREMER, Ecotoxicol Lab, F-17137 Lhoumeau, France.
2 : IFREMER, Fisheries Lab, F-17137 Lhoumeau, France.
3 : Univ Bordeaux 1, EPOC, UMR CNRS 5805, F-33405 Talence, France.
4 : INERIS, Direct Risques Chron, Unite Ecotoxicol Vitro & Vivo, F-60550 Verneuil En Halatte, France.
5 : Univ Rennes 1, Team NEED, Res Inst Hlth Environm & Occupat, INSERM U1085,Case 1302, F-35042 Rennes, France.
6 : Acad Sinica, Inst Mol Biol, Taipei, Taiwan.
7 : INRA LPGP, F-35042 Rennes, France.
Source Environmental Science And Pollution Research (0944-1344) (Springer Heidelberg), 2015-11 , Vol. 22 , N. 21 , P. 16371-16383
DOI 10.1007/s11356-015-4157-8
WOS© Times Cited 26
Note Danio rerio as a Model in Aquatic Toxicology and Sediment Research
Keyword(s) Danio rerio, Polycyclic aromatic hydrocarbon, Offspring, Transgenerational effect, Heavy oil, Light crude oil, Locomotor activity, Photomotor response, Anxiety-like behavior
Abstract The release of polycyclic aromatic hydrocarbons (PAHs) into the environment has increased very substantially over the last decades. PAHs are hydrophobic molecules which can accumulate in high concentrations in sediments acting then as major secondary sources. Fish contamination can occur through contact or residence nearby sediments or though dietary exposure. In this study, we analyzed certain physiological traits in unexposed fish (F1) issued from parents (F0) exposed through diet to three PAH mixtures at similar and environmentally relevant concentrations but differing in their compositions. For each mixture, no morphological differences were observed between concentrations. An increase in locomotor activity was observed in larvae issued from fish exposed to the highest concentration of a pyrolytic (PY) mixture. On the contrary, a decrease in locomotor activity was observed in larvae issued from heavy oil mixture (HO). In the case of the third mixture, light oil (LO), a reduction of the diurnal activity was observed during the setup of larval activity. Behavioral disruptions persisted in F1-PY juveniles and in their offspring (F2). Endocrine disruption was analyzed using cyp19a1b:GFP transgenic line and revealed disruptions in PY and LO offspring. Since no PAH metabolites were dosed in larvae, these findings suggest possible underlying mechanisms such as altered parental signaling molecule and/or hormone transferred in the gametes, eventually leading to early imprinting. Taken together, these results indicate that physiological disruptions are observed in offspring of fish exposed to PAH mixtures through diet.
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Vignet Caroline, Joassard Lucette, Lyphout Laura, Guionnet Tiphaine, Goubeau Manon, Le Menach Karyn, Brion Francois, Kah Olivier, Chung Bon-Chu, Budzinski Helene, Begout Marie-Laure, Cousin Xavier (2015). Exposures of zebrafish through diet to three environmentally relevant mixtures of PAHs produce behavioral disruptions in unexposed F1 and F2 descendant. Environmental Science And Pollution Research, 22(21), 16371-16383. Publisher's official version : https://doi.org/10.1007/s11356-015-4157-8 , Open Access version : https://archimer.ifremer.fr/doc/00259/37030/