A microarray-based analysis of oocyte quality in the European clam Ruditapes decussatus

Type Article
Date 2015-09
Language English
Author(s) De Sousa Joana Teixeira1, 1, 2, 2, Milan Massimo3, 3, Pauletto Marianna3, 3, Bargelloni Luca3, 3, Joaquim Sandra2, 2, 4, 4, Matias Domitilia2, 2, 4, 4, Matias Ana Margarete2, 2, Quillien Virgile1, 1, Leitao Alexandra2, 2, 5, 5, Huvet ArnaudORCID1, 1
Affiliation(s) 1 : IFREMER, Lab Sci Environm Marin, UMR CNRS 6539, F-29280 Plouzane, France.
2 : IPMA, P-8700305 Olhao, Portugal.
3 : Univ Padua, Dept Comparat Biomed & Food Sci, I-35020 Legnaro, Italy.
4 : Univ Porto, CIIMAR, Interdisciplinary Ctr Marine & Environm Res, P-4050123 Oporto, Portugal.
5 : Qatar Univ, Ctr Environm Studies, Doha, Qatar.
Source Aquaculture (0044-8486) (Elsevier Science Bv), 2015-09 , Vol. 446 , P. 17-24
DOI 10.1016/j.aquaculture.2015.04.018
WOS© Times Cited 6
Keyword(s) Ruditapes decussatus, Oocyte, Developmental success, Gene expression, Marine bivalve, Transcriptomics
Abstract A microarray-based analysis was performed with the objective of describing genomic features of oocytes and looking for potential markers of oocyte quality in the economically important European clam, Ruditapes decussatus. Oocytes of 25 females from Ria de Aveiro, Western coast of Portugal (40°42′N; 08°40′W) were selected for this study and oocyte quality was estimated by success of D-larval rate under controlled conditions, which appeared to vary from 0 to 95 %. By genome-wide expression profiling with a DNA microarray, 526 probes appeared differentially expressed between two groups representing the largest and smallest value of D larval rates, named good (represented by a mean D-larval yield of 57 ± 22%) and poor (9 ± 5%) quality oocytes. Enrichment analysis showed “Lysosome” (dre04142) as the single pathway represented in the enriched KEGG pathway terms, with 8 genes coding for putative Cathepsins. Furthermore, differentially expressed genes involved in oocyte protection (DnaJ (Hsp40), Hsp70, Cyclophilin B, PDI), maturation (Cam-PDE1C, PRDM9, G protein), sperm-egg interaction (PDI, G protein) and apoptosis (TNF) were also identified. From these, the apoptosis pathway was supposed to assume an important role in oocyte quality as the mRNA level of Caspase 8 appeared also negatively correlated with the D-larval yield. Finally, a G-protein transcript was identified as more abundant in poor quality oocytes, which underlines the importance of release prophase I block and meiotic maturation in the oocytes of this species. This study provided new highly valuable information on genes specifically expressed by mature oocytes of R. decussatus, for further understanding of the mechanisms of early development in this species.
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De Sousa Joana Teixeira, Milan Massimo, Pauletto Marianna, Bargelloni Luca, Joaquim Sandra, Matias Domitilia, Matias Ana Margarete, Quillien Virgile, Leitao Alexandra, Huvet Arnaud (2015). A microarray-based analysis of oocyte quality in the European clam Ruditapes decussatus. Aquaculture, 446, 17-24. Publisher's official version : https://doi.org/10.1016/j.aquaculture.2015.04.018 , Open Access version : https://archimer.ifremer.fr/doc/00260/37147/