Essential Fatty Acid Assimilation and Synthesis in Larvae of the Bivalve Crassostrea gigas
| Type | Article | ||||||||||||
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| Date | 2015-05 | ||||||||||||
| Language | English | ||||||||||||
| Author(s) | Da Costa Gonzalez Fiz1, 2, Robert Rene 1, 3, Quere Claudie1, Wikfors Gary H.4, Soudant Philippe5 |
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| Affiliation(s) | 1 : Ifremer/Laboratoire des sciences de l’Environnement Marin (UMR 6539, LEMAR), 29280, Plouzané, France 2 : Novostrea Bretagne, Route du Vieux Passage, Banastère, 56370, Sarzeau, France 3 : IFREMER, Unite Littoral, Ctr Bretagne, F-29280 Plouzane, France. 4 : NOAA, Northeast Fisheries Sci Ctr, NMFS, Milford, CT 06460 USA. 5 : IUEM UBO, LEMAR, Lab Sci Environm Marin UMR 6539, Plouzane, France. |
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| Source | Lipids (0024-4201) (Springer Heidelberg), 2015-05 , Vol. 50 , N. 5 , P. 503-511 | ||||||||||||
| DOI | 10.1007/s11745-015-4006-z | ||||||||||||
| WOS© Times Cited | 32 | ||||||||||||
| Keyword(s) | Fatty acid, Larvae, Lipids, Metabolism, Oyster, Synthesis | ||||||||||||
| Abstract | Essential fatty acids (EFA) are important for bivalve larval survival and growth. The purpose of this study was to quantitatively assess for the first time through a mass-balance approach dietary EFA incorporation and synthesis within Crassostrea gigas larvae. A first experiment was carried out using two microalgae, Tisochrysis lutea (T) and Chaetoceros neogracile (Cg), as mono- and bi-specific diets. A second experiment using a similar design was performed to confirm and extend the results obtained in the first. Flow-through larval rearing was used for accurate control of food supply and measurement of ingestion. Non-methylene-interrupted fatty acids were synthetized from precursors supplied in the diet: 16:1n-7 and 18:1n-9, mediated by Delta 5 desaturase. Moreover, this Delta 5 desaturase presumably allowed larvae to convert 20:3n-6 and 20:4n-3 to 20:4n-6 and 20:5n-3, respectively, when the product EFA were poorly or not supplied in the diet, as when larvae were fed T exclusively. Under our experimental conditions, none of the diets induced 22:6n-3 synthesis; however, 22:6n-3 incorporation into larval tissues occurred selectively under non-limiting dietary supply to maintain optimal levels in the larvae. This combination of flow-through larval rearing and biochemical analysis of FA levels could be applied to additional dietary experiments to precisely define optimal levels of EFA supply. | ||||||||||||
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