Interaction between toxic dinoflagellate Alexandrium catenella exposure and disease associated with herpesvirus OsHV-1μVar in Pacific oyster spat Crassostrea gigas

Type Article
Date 2015-05
Language English
Author(s) Lassudrie MalwennORCID1, Soudant Philippe1, Nicolas Jean-Louis2, Fabioux Caroline1, Lambert Christophe1, Miner Philippe2, Le Grand Jacqueline3, Petton BrunoORCID3, Hegaret HeleneORCID1
Affiliation(s) 1 : Inst Univ Europeen Mer, UMR CNRS UBO IRD IFREMER 6539, Lab Sci Environm Marin LEMAR, F-29280 Plouzane, France.
2 : IFREMER, Lab Sci Environm Marin LEMAR, IRD,CS 10070, Ctr Bretagne,ZI Pointe du Diable,CNRS,UMR UBO 653, F-29280 Plouzane, France.
3 : IFREMER, Lab Sci Environm Marin LEMAR, IRD, CNRS,UMR UBO 6539, F-29840 Argenton En Landunvez, France.
Source Harmful Algae (1568-9883) (Elsevier Science Bv), 2015-05 , Vol. 45 , P. 53-61
DOI 10.1016/j.hal.2015.04.007
WOS© Times Cited 22
Keyword(s) Crassostrea gigas, OsHV-1 mu Var, Harmful algal blooms, Alexandrium, Host-pathogen interaction, PST accumulation
Abstract Blooms of toxic dinoflagellates can co-occur with mass mortality events associated with herpesvirus OsHV-1 μVar infection that have been decimating Pacific oyster Crassostrea gigas spat and juveniles every summer since 2008 in France. This study investigated the possible effect of a harmful dinoflagellate, Alexandrium catenella, a producer of Paralytic Shellfish Toxins (PSTs), upon the oyster spat–herpesvirus interaction. Oyster spat from a hatchery were challenged by cohabitation with oysters contaminated in the field with OsHV-1 μVar and possibly other pathogens. Simultaneously, the oysters were exposed to cultured A. catenella. Infection with OsHV-1 μVar and PST accumulation were measured after 4 days of experimental exposure.

Exposure to Alexandrium catenella modified the host–pathogen interaction by reducing prevalence of OsHV-1 μVar infection. In addition, oysters challenged with OsHV-1 μVar and possibly other pathogens from the environment accumulated smaller amounts of PSTs than unchallenged oysters. Three possible mechanisms are suggested by these results: (i) possible direct interactions between A. catenella and herpesvirus (or associated pathogens) could reduce viral transmission and algal availability for oyster consumption; (ii) oyster feeding behavior or digestive functions may have been altered, thus decreasing both uptake of viral particles and consumption or digestion of toxic algae and consequent toxin accumulation; (iii) immuno-activation by A. catenella could enhance defense efficiency against OsHV-1 μVar infection. These findings suggest further research on relationships between OsHV-1 μVar and toxic dinoflagellates and their combined effects upon disease transmission and proliferation processes, as well as on oyster physiological and immunological involvement in this complex, tripartite interaction.
Full Text
File Pages Size Access
9 853 KB Access on demand
Author's final draft 40 1 MB Open access
Top of the page

How to cite 

Lassudrie Malwenn, Soudant Philippe, Nicolas Jean-Louis, Fabioux Caroline, Lambert Christophe, Miner Philippe, Le Grand Jacqueline, Petton Bruno, Hegaret Helene (2015). Interaction between toxic dinoflagellate Alexandrium catenella exposure and disease associated with herpesvirus OsHV-1μVar in Pacific oyster spat Crassostrea gigas. Harmful Algae, 45, 53-61. Publisher's official version : , Open Access version :