Rearing effect of biofloc on antioxidant and antimicrobial transcriptional response in Litopenaeus stylirostris shrimp facing an experimental sub-lethal hydrogen peroxide stress

Type Article
Date 2015-08
Language English
Author(s) Cardona Emilie1, 2, Saulnier Denis1, Lorgeoux Benedicte1, Chim Liet2, Gueguen YannickORCID1, 3
Affiliation(s) 1 : IFREMER, Unite Rech Resources Marines, Ctr Oceanolog Pacifique, Taravao 98719, Fr Polynesia.
2 : IFREMER, Unite Rech Lagons Ecosyst & Aquaculture Durable N, Noumea 98846, New Caledonia.
3 : Univ Montpellier, CNRS, UPVD, Ifremer,UMR IHPE 5244, F-34095 Montpellier, France.
Source Fish & Shellfish Immunology (1050-4648) (Academic Press Ltd- Elsevier Science Ltd), 2015-08 , Vol. 45 , N. 2 , P. 933-939
DOI 10.1016/j.fsi.2015.05.041
WOS© Times Cited 23
Keyword(s) Biofloc, Shrimps, Antimicrobial peptides, Antioxidant enzymes
Abstract This study compares the antioxidant and antimicrobial transcriptional expression of blue shrimps reared according to two different systems, BioFloc Technology (BFT) and Clear sea Water (CW) and their differential responses when facing an experimental sublethal hydrogen peroxide stress. After 30 days of rearing, juvenile shrimps were exposed to H2O2 stress at a concentration of 30 ppm during 6 hours. The oxidative stress caused by H2O2 was examined in the digestive glands of the shrimp, in which antioxidant enzyme (AOE) and antimicrobial peptide (AMP) gene expression were analysed by quantitative real-time PCR. Results showed that rearing conditions did not affect the expression of genes encoding AOEs or AMPs. However, H2O2 stress induced a differential response in expression between shrimps from the two rearing treatments (BFT and CW). Comparative analysis of the expression profiles indicates that catalase transcripts were significantly upregulated by H2O2 stress for BFT shrimps while no change was observed for CW shrimps. In contrast, H2O2 caused down-regulation of superoxide dismutase and glutathione transferase transcripts and of the three AMP transcripts studied (penaeidin 2 and 3, and crustin) for CW shrimps, while no effect was observed on BFT shrimp transcript levels. These results suggested that BFT shrimps maintained antioxidant and AMP responses after stress and therefore can effectively protect their cells against oxidative stress, while CW shrimp immune competence seems to decrease after stress.
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Cardona Emilie, Saulnier Denis, Lorgeoux Benedicte, Chim Liet, Gueguen Yannick (2015). Rearing effect of biofloc on antioxidant and antimicrobial transcriptional response in Litopenaeus stylirostris shrimp facing an experimental sub-lethal hydrogen peroxide stress. Fish & Shellfish Immunology, 45(2), 933-939. Publisher's official version : https://doi.org/10.1016/j.fsi.2015.05.041 , Open Access version : https://archimer.ifremer.fr/doc/00270/38116/