Vitamin A supplementation enhances Senegalese sole (Solea senegalensis) early juvenile’s immunocompetence: new insights on potential underlying pathways

Type Article
Date 2015-10
Language English
Author(s) Fernandez Ignacio1, 2, Lopez-Joven Maria-Carmen2, 3, Andree Karl B.2, Roque Ana2, Gisbert Enric2
Affiliation(s) 1 : Univ Algarve, Ctr Ciencias Mar CCMAR CIMAR LA, P-8005139 Faro, Portugal.
2 : Ctr St Carles de la Rapita IRTA SCR, IRTA, Unitat Cultius Expt, San Carlos de la Rapita 43540, Spain.
3 : Univ Perpignan, Univ Montpellier, IFREMER, IHPE,CNRS,UMR 5244, F-34095 Montpellier 5, France.
Source Fish & Shellfish Immunology (1050-4648) (Academic Press Ltd- Elsevier Science Ltd), 2015-10 , Vol. 46 , N. 2 , P. 703-709
DOI 10.1016/j.fsi.2015.08.007
WOS© Times Cited 8
Keyword(s) Vitamin, Retinoic acid, Immune system, Gene expression, Senegalese sole Solea senegalensis, Photobacterium damselae subp. damselae
Abstract Senegalese sole (Solea senegalensis) has been considered since the 1990´s to be a promising flatfish species for diversifying European marine aquaculture. However, pathogen outbreaks leading to high mortality rates can impair Senegalese sole commercial production at the weaning phase. Different approaches have been shown to improve fish immunocompetence; with this in mind the objective of the work described herein was to determine whether increased levels of dietary vitamin A (VA) improve the immune response in early juveniles of Senegalese sole. For this purpose, Senegalese sole were reared and fed with Artemia metanauplii containing increased levels of VA (37,000; 44,666; 82,666 and 203,000 total VA IU Kg-1) from 6 to 60 days post-hatch (early juvenile stage). After an induced bacterial infection with a 50 % lethal dose of Photobacterium damselae subsp. damselae, survival rate, as well as underlying gene expression of specific immune markers (C1inh, C3, C9, Lgals1, Hamp, LysC, Prdx1, Steap4 and Transf) were evaluated. Results showed that fish fed higher doses of dietary VA were more resistant to the bacterial challenge. The lower mortality was found to be related with differential expression of genes involved in the complement system and iron availability. We suggest that feeding metamorphosed Senegalese sole with 203,000 total VA IU Kg-1 might be an effective, inexpensive and environmentally friendly method to improve Senegalese sole immunocompetence, thereby improving survival of juveniles and reducing economic losses.
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Fernandez Ignacio, Lopez-Joven Maria-Carmen, Andree Karl B., Roque Ana, Gisbert Enric (2015). Vitamin A supplementation enhances Senegalese sole (Solea senegalensis) early juvenile’s immunocompetence: new insights on potential underlying pathways. Fish & Shellfish Immunology, 46(2), 703-709. Publisher's official version : https://doi.org/10.1016/j.fsi.2015.08.007 , Open Access version : https://archimer.ifremer.fr/doc/00275/38617/