Identification of genes associated with shell color in the black-lipped pearl oyster, Pinctada margaritifera

Type Article
Date 2015-08
Language English
Author(s) Lemer Sarah1, Saulnier Denis2, Gueguen YannickORCID2, Planes Serge1
Affiliation(s) 1 : USR 3278 CNRS CRIOBE EPHE, Lab Excellence CORAIL, Perpignan, France.
2 : IFREMER, Lab Excellence CORAIL, UMR EIO 241, Taravao 98719, Tahiti, Fr Polynesia.
Source Bmc Genomics (1471-2164) (Biomed Central Ltd), 2015-08 , Vol. 16 , N. 568 , P. 1-14
DOI 10.1186/s12864-015-1776-x
WOS© Times Cited 46
Keyword(s) Differential expression, Biomineralization, Nacre, Pearl, Pigmentation, Albino
Abstract Background: Color polymorphism in the nacre of pteriomorphian bivalves is of great interest for the pearl culture industry. The nacreous layer of the Polynesian black-lipped pearl oyster Pinctada margaritifera exhibits a large array of color variation among individuals including reflections of blue, green, yellow and pink in all possible gradients. Although the heritability of nacre color variation patterns has been demonstrated by experimental crossing, little is known about the genes involved in these patterns. In this study, we identify a set of genes differentially expressed among extreme color phenotypes of P. margaritifera using a suppressive and subtractive hybridization (SSH) method comparing black phenotypes with full and half albino individuals. Results: Out of the 358 and 346 expressed sequence tags (ESTs) obtained by conducting two SSH libraries respectively, the expression patterns of 37 genes were tested with a real-time quantitative PCR (RT-qPCR) approach by pooling five individuals of each phenotype. The expression of 11 genes was subsequently estimated for each individual in order to detect inter-individual variation. Our results suggest that the color of the nacre is partially under the influence of genes involved in the biomineralization of the calcitic layer. A few genes involved in the formation of the aragonite tablets of the nacre layer and in the biosynthesis chain of melanin also showed differential expression patterns. Finally, high variability in gene expression levels were observed within the black phenotypes. Conclusions: Our results revealed that three main genetic processes were involved in color polymorphisms: the biomineralization of the nacreous and calcitic layers and the synthesis of pigments such as melanin, suggesting that color polymorphism takes place at different levels in the shell structure. The high variability of gene expression found within black phenotypes suggests that the present work should serve as a basis for future studies exploring more thoroughly the expression patterns of candidate genes within black phenotypes with different dominant iridescent colors.
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