Effect of Nitrate, Ammonium and Urea on Growth and Pinnatoxin G Production of Vulcanodinium rugosum

Type Article
Date 2015-09
Language English
Author(s) Abadie EricORCID1, Kaci Lamia1, Berteaux Tom1, Hess PhilippORCID2, Sechet VeroniqueORCID2, Masseret Estelle3, Rolland Jean-Luc4, Laabir Mohamed3
Affiliation(s) 1 : IFREMER, Lab Environm Ressources Languedoc Roussillon, Ctr Marine Biodivers Exploitat & Conservat MARBEC, Sete 3, France.
2 : IFREMER, Dept ODE UL PHYC, F-44311 Nantes 3, France.
3 : Univ Montpellier, Ctr Marine Biodivers Exploitat & Conservat MARBEC, CNRS, IRD, F-34095 Montpellier 5, France.
4 : Univ Montpellier, Univ Perpignan, IFREMER, Interact Hotes Pathogenes Environm,UMR 5244, F-34095 Montpellier, France.
Source Marine Drugs (1660-3397) (Mdpi Ag), 2015-09 , Vol. 13 , N. 9 , P. 5642-5656
DOI 10.3390/md13095642
WOS© Times Cited 9
Keyword(s) Vulcanodinium rugosum, pinnatoxin G, Ingril lagoon, growth, nitrogen source
Abstract ulcanodinium rugosum, a recently described dinoflagellate species producing a potent neurotoxin (pinnatoxin G), has been identified in French Mediterranean lagoons and was responsible for recurrent episodes of shellfish toxicity detected by mouse bioassay. Until now, the biology and physiology of V. rugosum have not been fully investigated. We studied the growth characteristics and toxicity of a V. rugosum strain (IFR-VRU-01), isolated in the Ingril lagoon in June 2009 (North-Western French Mediterranean Sea). It was cultivated in Enriched Natural Sea Water (ENSW) with organic (urea) and inorganic (ammonium and nitrate) nitrogen, at a temperature of 25 °C and irradiance of 100 μmol/m2·s−1. Results showed that ammonium was assimilated by cells more rapidly than nitrate and urea. V. rugosum is thus an osmotrophic species using urea. Consequently, this nitrogen form could contribute to the growth of this dinoflagellate species in the natural environment. There was no significant difference (Anova, p = 0.856) between the growth rate of V. rugosum cultivated with ammonium (0.28 ± 0.11 day−1), urea (0.26 ± 0.08 day−1) and nitrate (0.24 ± 0.01 day−1). However, the production of chlorophyll a and pinnatoxin G was significantly lower with urea as a nitrogen source (Anova, p < 0.027), suggesting that nutritional conditions prevailing at the moment of the bloom could determine the cellular toxicity of V. rugosum and therefore the toxicity measured in contaminated mollusks. The relatively low growth rate (≤0.28 day−1) and the capacity of this species to continuously produce temporary cysts could explain why cell densities of this species in the water column are typically low (≤20,000 cells/L).
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Abadie Eric, Kaci Lamia, Berteaux Tom, Hess Philipp, Sechet Veronique, Masseret Estelle, Rolland Jean-Luc, Laabir Mohamed (2015). Effect of Nitrate, Ammonium and Urea on Growth and Pinnatoxin G Production of Vulcanodinium rugosum. Marine Drugs, 13(9), 5642-5656. Publisher's official version : https://doi.org/10.3390/md13095642 , Open Access version : https://archimer.ifremer.fr/doc/00276/38748/