Metabolic responses of clam Ruditapes philippinarum exposed to its pathogen Vibrio tapetis in relation to diet

Type Article
Date 2016-07
Language English
Author(s) Richard Gaelle1, Guerard Fabienne1, Corporeau Charlotte2, Lambert Christophe1, Paillard Christine1, Pernet FabriceORCID2
Affiliation(s) 1 : Univ Bretagne Occidentale, Inst Univ Europeen Mer, LEMAR IUEM UBO, UMR CNRS UBO IRD IFREMER 6539, Technopole Brest Iroise,Rue Dumont dUrville, F-29280 Plouzane, France.
2 : IFREMER, UMR LEMAR CNRS UBO IRD Ifremer 6539, Technopole Brest Iroise CS 10070, F-29280 Plouzane, France.
Source Developmental And Comparative Immunology (0145-305X) (Elsevier Sci Ltd), 2016-07 , Vol. 60 , P. 96-107
DOI 10.1016/j.dci.2016.02.026
WOS© Times Cited 5
Keyword(s) Antioxidant enzymes, Immunity, Energetic metabolism, Ruditapes philippinarum, Vibrio tapetis
Abstract We investigated the effect of brown ring disease (BRD)1 development and algal diet on energy reserves and activity of enzymes related to energy metabolism, antioxidant system and immunity in Manila clam, Ruditapes philippinarum. We found that algal diet did not impact the metabolic response of clams exposed to Vibrio tapetis. At two days post-injection (dpi), activities of superoxide dismutase and glutathione peroxidase (GPx) decreased whereas activities of nitric oxide synthase (iNOS) and catalase increased in infected clams, although no clinical signs were visible (BRD-). At 7 dpi, activities of several antioxidant and immune-related enzymes were markedly increased in BRD-likely indicating an efficient reactive oxygen species (ROS) scavenging compared to animals which developed clinical signs of BRD (BRD+). Therefore, resistance to BRD clinical signs appearance was associated with higher detoxification of ROS and enhancement of immune response. This study provides new biochemical indicators of disease resistance and a more comprehensive view of the global antioxidant response of clam to BRD development.
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Richard Gaelle, Guerard Fabienne, Corporeau Charlotte, Lambert Christophe, Paillard Christine, Pernet Fabrice (2016). Metabolic responses of clam Ruditapes philippinarum exposed to its pathogen Vibrio tapetis in relation to diet. Developmental And Comparative Immunology, 60, 96-107. Publisher's official version : https://doi.org/10.1016/j.dci.2016.02.026 , Open Access version : https://archimer.ifremer.fr/doc/00316/42684/