Does broodstock nutritional history affect the response of progeny to different first-feeding diets? A whole-body transcriptomic study of rainbow trout alevins

Type Article
Date 2016-06
Language English
Author(s) Lazzarotto Viviana1, Corraze Genevieve1, Larroquet Laurence1, Mazurais DavidORCID2, Medale Francoise1
Affiliation(s) 1 : INRA, UR Nutr Metab Aquaculture 1067, Aquapole, St Pee Sur Nivelle, France.
2 : IFREMER, IRD, UBO, CNRS,LEMAR UMR 6539,ZI Pointe Diable,CS 10070, F-29280 Plouzane, France.
Source British Journal Of Nutrition (0007-1145) (Cambridge Univ Press), 2016-06 , Vol. 115 , N. 12 , P. 2079-2092
DOI 10.1017/S0007114516001252
WOS© Times Cited 29
Keyword(s) Fish, Nutrition, Plant products, Fatty acids, Gene expression, Microarrays, Early stages
Abstract The whole-body transcriptome of trout alevins was characterised to investigate the effects of long-term feeding of rainbow trout broodstock females a diet free of fishmeal and fish oil on the metabolic capacities of progeny. Effects were studied before first feeding and after 3 weeks of feeding diets containing different proportions of marine and plant ingredients. Feeding alevins plant-based diets resulted in lower fish body weight, irrespective of maternal nutritional history. No differences in whole-body lipids were found between treatments, and the tissue fatty acid profile strongly reflected that of the respective broodstock or first-feeding diets. We showed that the maternal diet history did not significantly affect expressions of any genes before the first feeding. Interestingly, we found an effect of maternal nutritional history on gene expression in alevins after 3 weeks of feeding. The major differences in the transcriptome of alevins from plant-based diet-fed females compared with those from commercial-fed females were as follows: (i) down-regulation of genes involved in muscle growth/contraction and (ii) up-regulation of genes involved in carbohydrate and energy metabolism related to the delay in growth/development observed with plant-based diets. Our findings also showed an effect of the first-feeding diets, irrespective of maternal nutritional history. Specifically, the introduction of plant ingredients resulted in the up-regulation of genes involved in amino acid/protein and cholesterol metabolism and in differences in the expressions of genes related to carbohydrate metabolism. Information gained through this study opens up avenues for further reduction of marine ingredients in trout diets, including the whole rearing cycle.
Full Text
File Pages Size Access
Author's final draft 23 1 MB Open access
Supplementary material 9 206 KB Open access
Figure S1 94 KB Open access
Figure S2-S2a 135 KB Open access
Figure S2b 132 KB Open access
Figure S3-S3a 184 KB Open access
Figure S3b 99 KB Open access
14 1 MB Access on demand
Top of the page

How to cite 

Lazzarotto Viviana, Corraze Genevieve, Larroquet Laurence, Mazurais David, Medale Francoise (2016). Does broodstock nutritional history affect the response of progeny to different first-feeding diets? A whole-body transcriptomic study of rainbow trout alevins. British Journal Of Nutrition, 115(12), 2079-2092. Publisher's official version : , Open Access version :