Bacterial community characterization of water and intestine of the shrimp Litopenaeus stylirostris in a biofloc system

Type Article
Date 2016-07
Language English
Author(s) Cardona Emilie1, 2, Gueguen YannickORCID1, 4, Magre Kevin1, Lorgeoux Benedicte1, Piquemal David3, Pierrat Fabien3, Noguier Florian3, Saulnier Denis1
Affiliation(s) 1 : IFREMER, UMR EIO 241, UPF ILM IRD, Labex Corail, BP 7004, Taravao 98719, Tahiti, Fr Polynesia.
2 : IFREMER, Unite Rech Lagons Ecosyst & Aquaculture Durable N, Noumea, New Caledonia.
3 : ACOBIOM, 1682 Rue Valsiere,Cap Delta CS77394, F-34184 Montpellier, France.
4 : Univ Montpellier, IFREMER, CNRS, UMR IHPE 5244,UPVD, F-34095 Montpellier, France.
Source Bmc Microbiology (1471-2180) (Biomed Central Ltd), 2016-07 , Vol. 16 , N. 157 , P. 9p.
DOI 10.1186/s12866-016-0770-z
WOS© Times Cited 113
Keyword(s) Shrimp, Biofloc, Clear sea water, Bacterial communities, Culture environment, Microbiota
Abstract Background

Biofloc technology (BFT), a rearing method with little or no water exchange, is gaining popularity in aquaculture. In the water column, such systems develop conglomerates of microbes, algae and protozoa, together with detritus and dead organic particles. The intensive microbial community presents in these systems can be used as a pond water quality treatment system, and the microbial protein can serve as a feed additive. The current problem with BFT is the difficulty of controlling its bacterial community composition for both optimal water quality and optimal shrimp health. The main objective of the present study was to investigate microbial diversity of samples obtained from different culture environments (Biofloc technology and clear seawater) as well as from the intestines of shrimp reared in both environments through high-throughput sequencing technology.
Results

Analyses of the bacterial community identified in water from BFT and “clear seawater” (CW) systems (control) containing the shrimp Litopenaeus stylirostris revealed large differences in the frequency distribution of operational taxonomic units (OTUs). Four out of the five most dominant bacterial communities were different in both culture methods. Bacteria found in great abundance in BFT have two principal characteristics: the need for an organic substrate or nitrogen sources to grow and the capacity to attach to surfaces and co-aggregate. A correlation was found between bacteria groups and physicochemical and biological parameters measured in rearing tanks. Moreover, rearing-water bacterial communities influenced the microbiota of shrimp. Indeed, the biofloc environment modified the shrimp intestine microbiota, as the low level (27 %) of similarity between intestinal bacterial communities from the two treatments.
Conclusion

This study provides the first information describing the complex biofloc microbial community, which can help to understand the environment-microbiota-host relationship in this rearing system.
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Cardona Emilie, Gueguen Yannick, Magre Kevin, Lorgeoux Benedicte, Piquemal David, Pierrat Fabien, Noguier Florian, Saulnier Denis (2016). Bacterial community characterization of water and intestine of the shrimp Litopenaeus stylirostris in a biofloc system. Bmc Microbiology, 16(157), 9p. Publisher's official version : https://doi.org/10.1186/s12866-016-0770-z , Open Access version : https://archimer.ifremer.fr/doc/00346/45694/