Colonization of polystyrene microparticles by Vibrio crassostreae: light and electron microscopic investigation

Type Article
Date 2016-10
Language English
Author(s) Foulon Valentin1, Le Roux Frederique2, 3, Lambert Christophe1, Huvet ArnaudORCID4, Soudant Philippe1, Paul-Pont Ika1
Affiliation(s) 1 : Inst Univ Europeen Mer, UMR CNRS UBO IRD Ifremer 6539, Lab Sci Environm Marin LEMAR, Technopole Brest Iroise, Rue Dumont Urville, F-29280 Plouzane, France.
2 : Ifremer, Unite Physiol Fonct Organismes Marins, ZI Pointe Diable, CS 10070, F-29280 Plouzane, France.
3 : UPMC Paris 06, Sorbonne Univ, CNRS, UMR 8227,Integrat Biol Marine Models,Stn Biol Ros, CS 90074, F-29688 Roscoff, France.
4 : Ctr Bretagne, ZI Pointe Diable, UMR UBO CNRS IRD Ifremer 6539, Ifremer,Lab Sci Environm Marin LEMAR, CS 10070, F-29280 Plouzane, France.
Source Environmental Science & Technology (0013-936X) (Amer Chemical Soc), 2016-10 , Vol. 50 , N. 20 , P. 10988-10996
DOI 10.1021/acs.est.6b02720
WOS© Times Cited 25
Abstract Microplastics collected at sea harbour a high diversity of microorganisms including some Vibrio genus members, raising questions about the role of microplastics as a novel ecological niche for potentially pathogenic microorganisms. In the present study we investigated the adhesion dynamics of Vibrio crassostreae on polystyrene microparticles (micro-PS) using electronic and fluorescence microscopy techniques. Micro-PS were incubated with bacteria in different media (Zobell culture medium and artificial seawater) with or without natural marine aggregates. The highest percentage of colonised particles (38-100%) was observed in Zobell culture medium, which may be related to nutrient availability for production of pili and exopolysaccharide adhesion structures. A longer bacterial attachment (6 days) was observed on irregular micro-PS compared to smooth particles (<10h) but complete decolonisation of all particles eventually occurred. The presence of natural marine agreggates around micro-PS led to substantial and perennial colonisation featuring monospecific biofilms at the surface of the aggregates. These exploratory results suggest that V. crassostreae may be a secondary coloniser of micro-PS, requiring a multi-species community to form a durable adhesion phenotype. Temporal assessment of microbial colonisation on microplastics at sea using imaging and omics approaches are further indicated to better understand the microplastics colonisation dynamics and species assemblages.
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Foulon Valentin, Le Roux Frederique, Lambert Christophe, Huvet Arnaud, Soudant Philippe, Paul-Pont Ika (2016). Colonization of polystyrene microparticles by Vibrio crassostreae: light and electron microscopic investigation. Environmental Science & Technology, 50(20), 10988-10996. Publisher's official version : https://doi.org/10.1021/acs.est.6b02720 , Open Access version : https://archimer.ifremer.fr/doc/00351/46210/