Digital PCR for Quantifying Norovirus in Oysters Implicated in Outbreaks, France

Polo, David; Schaeffer, Julien; Fournet, Nelly; Le Saux, Jean-Claude; Parnaudeau, Sylvain; McLeod, Catherine; Le Guyader, Soizick

doi:10.3201/eid2212.160841

Digital PCR for Quantifying Norovirus in Oysters Implicated in Outbreaks, France

Using samples from oysters clearly implicated in human disease, we quantified norovirus levels by using digital PCR. Concentrations varied from 43 to 1,170 RNA copies/oyster. The analysis of frozen samples from the production area showed the presence of norovirus 2 weeks before consumption.

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Polo David, Schaeffer Julien, Fournet Nelly, Le Saux Jean-Claude, Parnaudeau Sylvain, McLeod Catherine, Le Guyader Soizick (2016). Digital PCR for Quantifying Norovirus in Oysters Implicated in Outbreaks, France. Emerging Infectious Diseases. 22 (12). 2189-2191. https://doi.org/10.3201/eid2212.160841, https://archimer.ifremer.fr/doc/00359/46971/

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