Adsorption of norovirus and ostreid herpesvirus type 1 to polymer membranes for the development of passive samplers
|Hubert Francoise1, Morga Benjamin2, Renault Tristan3, Le Guyader Soizick1
|1 : IFREMER, LSEM SG2M, Lab Microbiol, Rue Ile Yeu,BP 21105, F-44311 Nantes 03, France.
2 : SG2M Stn La Tremblade, Lab Genet & Pathol Mollusques, La Tremblade, France.
3 : IFREMER, Dept Ressources Biol & Environm, Nantes, France.
|Journal Of Applied Microbiology (1364-5072) (Wiley), 2017-04 , Vol. 122 , N. 4 , P. 1039-1047
|WOS© Times Cited
|norovirus, OsHV-1, Pacific oysters, passive samplers, seawater, sewage
|AimsThis study was performed to develop passive sampling methodology for the detection of two viruses in seawater in the area of shellfish production, The Norovirus (NoV), a human pathogen implicated in gastroenteritis outbreaks linked to oyster consumption and the ostreid herpesvirus type 1 (OsHV-1) a virus associated with mass mortalities of Pacific oysters.
Methods and ResultsCommercially membranes were tested for their capacity to adsorb virus: Zetapor, gauze, nylon, low density polyethylene (LDPE) and Polyvinylidene difluoride (PVDF). Laboratory exposures of membranes to contaminated water samples (stool, sewage, seawater) were performed. Our data shown that the amount of NoV GII genome per membrane measured with qRT-PCR increased with the time of exposure up to 24h, for all types of membranes except gauze. After 15 days of exposure, the amount of NoV GII per membrane continued to increase only for nylon and LDPE. The amount of OsHV-1 per zetapor membrane was significantly increased as soon as 4h of exposure, and after 24 h of exposure for all types of membranes. Exposure of membranes to serial dilutions of various samples revealed that the amount of NoV GII and OsHV-1 per membrane is significantly higher in diluted samples. The detection of NoV and OsHV-1 respectively with zetapor and PVDF membranes were found to be more efficient than direct analysis of sewage and sea water Conclusions: All membranes immerged in contaminated samples adsorbed NoV GII and OsHV-1. The amount of both virus increased with the time of exposure. Zetapor and PVDF membranes seems to be more adapted to NoV GII and OsHV-1 detection respectively.
Significance and Impact of the studyMembranes tested will be used as passive samplers to improve the detection of virus in oyster production areas. Also, passive samplers could be a valuable tool for microbiome analysis with New Generation Sequencing.