An updated assessment of Symbiodinium spp. that associate with common scleractinian corals from Moorea (French Polynesia) reveals high diversity among background symbionts and a novel finding of clade B

Type Article
Date 2017-01
Language English
Author(s) Rouze Heloise1, Lecellier Gael J.1, 2, Saulnier Denis3, Planes Serge1, Gueguen YannickORCID4, Wirshing Herman H.5, Berteaux-Lecellier Veronique1
Affiliation(s) 1 : Labex CORAIL, PSL CRIOBE CNRS EPHE UPVD USR3278, Papetoai, Moorea, Fr Polynesia.
2 : Univ Paris Saclay, Dept Biol, Versailles, France.
3 : Labex CORAIL, EIO Ifremer ILM IRD UPF UMR241, Taravao, Fr Polynesia.
4 : Univ Montpellier, CNRS Ifremer UM UPVD, IHPE UMR5244, Montpellier, France.
5 : Smithsonian Natl Museum Nat Hist, Dept Invertebrate Zool, Washington, DC USA.
Source Peerj (2167-8359) (Peerj Inc), 2017-01 , Vol. 5 , P. e2856 (1-22)
DOI 10.7717/peerj.2856
WOS© Times Cited 14
Keyword(s) Corals, French polynesia, Clade B, Symbiodinium, qPCR, Flexibility, Generalist, Faithful clade
Abstract The adaptative bleaching hypothesis (ABH) states that, depending on the symbiotic flexibility of coral hosts (i.e., the ability of corals to “switch” or “shuffle” their algal symbionts), coral bleaching can lead to a change in the composition of their associated Symbiodinium community and, thus, contribute to the coral’s overall survival. In order to determine the flexibility of corals, molecular tools are required to provide accurate species delineations and to detect low levels of coral-associated Symbiodinium. Here, we used highly sensitive quantitative (real-time) PCR (qPCR) technology to analyse five common coral species from Moorea (French Polynesia), previously screened using only traditional molecular methods, to assess the presence of low-abundance (background) Symbiodinium spp. Similar to other studies, each coral species exhibited a strong specificity to a particular clade, irrespective of the environment. In addition, however, each of the five species harboured at least one additional Symbiodinium clade, among clades A–D, at background levels. Unexpectedly, and for the first time in French Polynesia, clade B was detected as a coral symbiont. These results increase the number of known coral-Symbiodinium associations from corals found in French Polynesia, and likely indicate an underestimation of the ability of the corals in this region to associate with and/or “shuffle” different Symbiodinium clades. Altogether our data suggest that corals from French Polynesia may favor a trade-off between optimizing symbioses with a specific Symbiodinium clade(s), maintaining associations with particular background clades that may play a role in the ability of corals to respond to environmental change.
Full Text
File Pages Size Access
Publisher's official version 22 2 MB Open access
Supplementary methods 2 169 KB Open access
Raw datas: qPCR results 34 KB Open access
Efficiency of specific-clade primer sets 1 366 KB Open access
Efficiency of specific-clade primer sets on Symbiodinium cells isolated from corals 1 121 KB Open access
Phylogenetic tree of Symbiodinium using sequences of 28S rDNA 1 392 KB Open access
Symbiodinium clades C and F in seawater samples of Moorea 1 121 KB Open access
Characteristics of primer sets 1 163 KB Open access
qPCR assays on cultures Symbiodinium strains 1 343 KB Open access
Symbiodinium 28S DNA sequences mentioned in the paper 2 205 KB Open access
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Rouze Heloise, Lecellier Gael J., Saulnier Denis, Planes Serge, Gueguen Yannick, Wirshing Herman H., Berteaux-Lecellier Veronique (2017). An updated assessment of Symbiodinium spp. that associate with common scleractinian corals from Moorea (French Polynesia) reveals high diversity among background symbionts and a novel finding of clade B. Peerj, 5, e2856 (1-22). Publisher's official version : https://doi.org/10.7717/peerj.2856 , Open Access version : https://archimer.ifremer.fr/doc/00365/47652/