Development of a qPCR Method for the Identification and Quantification of Two Closely Related Tuna Species, Bigeye Tuna ( Thunnus obesus ) and Yellowfin Tuna ( Thunnus albacares ), in Canned Tuna
|Author(s)||Bojolly Daline1, 6, 8, Doyen Perine1, 2, 3, 5, Le Fur Bruno8, Christaki Urania6, Verrez-Bagnis Veronique7, Grard Thierry1, 2, 3, 4, 5|
|Affiliation(s)||1 : Univ Littoral Cote dOpale, EA 7394, ICV, USC Anses ULCO, F-62200 Boulogne Sur Mer, France.
2 : Univ Lille, F-59000 Lille, France.
3 : Univ Artois, F-62000 Arras, France.
4 : INRA, Paris, France.
5 : ISA, F-59000 Lille, France.
6 : Lille 1, ULCO, CNRS, Lab Oceanol & Geosci,UMR 8187, F-62930 Wimereux, France.
7 : IFREMER, F-44300 Nantes, France.
8 : PFINV, F-62200 Boulogne Sur Mer, France.
|Source||Journal Of Agricultural And Food Chemistry (0021-8561) (Amer Chemical Soc), 2017-02 , Vol. 65 , N. 4 , P. 913-920|
|WOS© Times Cited||15|
|Keyword(s)||tuna, authentication, quantification, canned products, TaqMan, qPCR, species identification, bigeye tuna (Thunnus obesus), yellowfin tuna (Thunnus albacares)|
|Abstract||Bigeye tuna (Thunnus obesus) and yellowfin tuna (Thunnus albacares) are among the most widely used tuna species for canning purposes. Not only substitution but also mixing of tuna species is prohibited by the European regulation for canned tuna products. However, as juveniles of bigeye and yellowfin tunas are very difficult to distinguish, unintentional substitutions may occur during the canning process. In this study, two mitochondrial markers from NADH dehydrogenase subunit 2 and cytochrome c oxidase subunit II genes were used to identify bigeye tuna and yellowfin tuna, respectively, utilizing TaqMan qPCR methodology. Two different qPCR-based methods were developed to quantify the percentage of flesh of each species used for can processing. The first one was based on absolute quantification using standard curves realized with these two markers; the second one was founded on relative quantification with the universal 12S rRNA gene as the endogenous gene. On the basis of our results, we conclude that our methodology could be applied to authenticate these two closely related tuna species when used in a binary mix in tuna cans.|