High-density genetic map and identification of QTLs for responses to temperature and salinity stresses in the model brown alga Ectocarpus

Type Article
Date 2017-03
Language English
Author(s) Avia Komlan1, 2, Coelho Susana M.1, Montecinos Gabriel J.1, 2, 3, 4, Cormier Alexandre1, Lerck Fiona1, Mauger Stephane2, Faugeron Sylvain2, 3, 4, Valero Myriam2, Cock J. Mark1, Boudry PierreORCID5
Affiliation(s) 1 : UPMC, Sorbonne Univ, CNRS, Algal Genet Grp,UMR 8227,Stn Biol Roscoff, CS 90074, F-29688 Roscoff, France.
2 : UPMC, Sorbonne Univ, Pontificia Univ Catolica Chile,Stn Biol Roscoff, Univ Austral Chile,CNRS,Evolut Biol & Ecol Algae, CS 90074, F-29688 Roscoff, France.
3 : Pontificia Univ Catolica Chile, Fac Ciencias Biol, Ctr Conservac Marina, Casilla 114-D, Santiago, Chile.
4 : Pontificia Univ Catolica Chile, Fac Ciencias Biol, CeBiB, Casilla 114-D, Santiago, Chile.
5 : UBO, CNRS, IRD,LEMAR,UMR 6539, Ifremer,Ctr Bretagne ZI Pointe Diable,Lab Sci Env, CS 10070, F-29280 Plouzane, France.
Source Scientific Reports (2045-2322) (Nature Publishing Group), 2017-03 , Vol. 7 , N. 43241 , P. 1-15
DOI 10.1038/srep43241
WOS© Times Cited 36
Abstract Deciphering the genetic architecture of adaptation of brown algae to environmental stresses such as temperature and salinity is of evolutionary as well as of practical interest. The filamentous brown alga Ectocarpus sp. is a model for the brown algae and its genome has been sequenced. As sessile organisms, brown algae need to be capable of resisting the various abiotic stressors that act in the intertidal zone (e.g. osmotic pressure, temperature, salinity, UV radiation) and previous studies have shown that an important proportion of the expressed genes is regulated in response to hyposaline, hypersaline or oxidative stress conditions. Using the double digest RAD sequencing method, we constructed a dense genetic map with 3,588 SNP markers and identified 39 QTLs for growth-related traits and their plasticity under different temperature and salinity conditions (tolerance to high temperature and low salinity). GO enrichment tests within QTL intervals highlighted membrane transport processes such as ion transporters. Our study represents a significant step towards deciphering the genetic basis of adaptation of Ectocarpus sp. to stress conditions and provides a substantial resource to the increasing list of tools generated for the species.
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Avia Komlan, Coelho Susana M., Montecinos Gabriel J., Cormier Alexandre, Lerck Fiona, Mauger Stephane, Faugeron Sylvain, Valero Myriam, Cock J. Mark, Boudry Pierre (2017). High-density genetic map and identification of QTLs for responses to temperature and salinity stresses in the model brown alga Ectocarpus. Scientific Reports, 7(43241), 1-15. Publisher's official version : https://doi.org/10.1038/srep43241 , Open Access version : https://archimer.ifremer.fr/doc/00377/48779/