Inter and intra-specific growth and domoic acid production in relation to nutrient ratios and concentrations in Pseudo-nitzschia : phosphate an important factor

Type Article
Date 2017-04
Language English
Author(s) Lema Anais Kimberley1, Latimier Marie1, Nezan Elisabeth2, Fauchot Juliette3, 4, Le Gac Mickael1
Affiliation(s) 1 : IFREMER, DYNECO PELAGOS, F-29280 Plouzane, France.
2 : IFREMER, Stn Biol Marine, Pl Croix,BP 40537, F-29185 Concarneau, France.
3 : Normandie Univ, UNICAEN, F-14000 Caen, France.
4 : UCBN, UPMC, MNHN, CNRS 7208,IRD 207,UMR BORER, F-14032 Caen, France.
Source Harmful Algae (1568-9883) (Elsevier Science Bv), 2017-04 , Vol. 64 , P. 11-19
DOI 10.1016/j.hal.2017.03.001
WOS© Times Cited 12
Keyword(s) Pseudo-nitzschia strains, Domoic acid, Ratio, Phosphate, Nitrogen
Abstract

The factors responsible for inducing the synthesis of toxins and responses from toxic phytoplankton blooms remain unclear. In this study we compare the influence of genotypic (at both the intra and interspecific levels) and environmental factors (nutrient concentration and ratio) on growth (in terms of cell densities) and domoic acid (DA) production in three Pseudo-nitzschia species: P. australis, P.pungens and P.fradulenta. A strong phosphate effect was detected. More precisely, a low initial concentration in phosphate, even at high initial nitrogen and silicate concentrations, induced the highest DA concentrations and the lowest cell densities in all strains/species studied. In contrast, a low initial concentration of nitrogen and silicate combined, with a higher phosphate concentration resulted in low cell densities, but without high DA production. Inter-species effects were also observed in DA production, where P. australis represented the most toxigenic species of all. Intra-specific variations were only moderate, except for a recently isolated P. australis strain, suggesting the influence of time since isolation on the physiology and DA production of Pseudo-nitzschia species. Overall, the lack of strong interaction between environmental and genotypic factors showed that the various genotypes investigated did not extensively diverge in their ability to respond (in terms of DA production and cell densities) to contrasting nutrient supply.

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Lema Anais Kimberley, Latimier Marie, Nezan Elisabeth, Fauchot Juliette, Le Gac Mickael (2017). Inter and intra-specific growth and domoic acid production in relation to nutrient ratios and concentrations in Pseudo-nitzschia : phosphate an important factor. Harmful Algae, 64, 11-19. Publisher's official version : https://doi.org/10.1016/j.hal.2017.03.001 , Open Access version : https://archimer.ifremer.fr/doc/00377/48849/