Parental diuron-exposure alters offspring transcriptome and fitness in Pacific oyster Crassostrea gigas

Type Article
Date 2017-08
Language English
Author(s) Bachere Evelyne1, Barranger Audrey2, Bruno Roman3, Rouxel Julien2, Menard Dominique2, Piquemal David3, 4, Akcha Farida2
Affiliation(s) 1 : Univ Montpellier, IFREMER, CNRS,UMR 5244, IHPE Interact Hosts Pathogens Environm,UPVD,CC 80, F-34095 Montpellier, France.
2 : IFREMER, Lab Ecotoxicol, Rue Ile Yeu,BP21105, F-44311 Nantes 03, France.
3 : Acobiom, 1682 Rue Valsiere, F-34184 Montpellier 04, France.
4 : Diag4Zoo, 1 Rue Loutres, F-34170 Montpellier, France.
Source Ecotoxicology And Environmental Safety (0147-6513) (Academic Press Inc Elsevier Science), 2017-08 , Vol. 142 , P. 51-58
DOI 10.1016/j.ecoenv.2017.03.030
WOS© Times Cited 16
Keyword(s) Marine bivalve, Herbicide, Toxicogenomics, Survival, Biomarkers, Global DNA-methylation
Abstract

One of the primary challenges in ecotoxicology is to contribute to the assessment of the ecological status of ecosystems. In this study, we used Pacific oyster Crassostrea gigas to explore the effects of a parental exposure to diuron, a herbicide frequently detected in marine coastal environments. The present toxicogenomic study provides evidence that exposure of oyster genitors to diuron during gametogenesis results in changes in offspring, namely, transcriptomic profile alterations, increased global DNA methylation levels and reduced growth and survival within the first year of life. Importantly, we highlighted the limitations to identify particular genes or gene expression signatures that could serve as biomarkers for parental herbicide-exposure and further for multigenerational and transgenerational effects of specific chemical stressors. By analyzing samples from two independent experiments, we demonstrated that, due to complex confounding effects with both tested solvent vehicles, diuron non-specifically affected the offspring transcriptome. These original results question the potential development of predictive genomic tools for detecting specific indirect impacts of contaminants in environmental risk assessments. However, our results indicate that chronic environmental exposure to diuron over several generations may have significant long term impacts on oyster populations with adverse health outcomes.

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