Colonization of plant substrates at hydrothermal vents and cold seeps in the northeast Atlantic and Mediterranean and occurrence of symbiont-related bacteria

Type Article
Date 2015-02
Language English
Author(s) Szafranski Kamil M.1, 2, Deschamps Philippe3, Cunha Marina R.4, 5, Gaudron Sylvie M.1, 2, Duperron Sebastien1, 2, 6
Affiliation(s) 1 : Univ Paris 06, Sorbonne Univ, UMR 7208, Adaptat Milieux Extremes, F-75005 Paris, France.
2 : UMR MNHN UPMC CNRS IRD UCBN 7208, Biol Organismes Aquat & Ecosyst, Paris, France.
3 : Univ Paris 11, CNRS, UMR8079, Unite Ecol Systemat & Evolut, F-91405 Orsay, France.
4 : Univ Aveiro, Dept Biol, P-3800 Aveiro, Portugal.
5 : Univ Aveiro, CESAM, P-3800 Aveiro, Portugal.
6 : Inst Univ France, Paris, France.
Source Frontiers In Microbiology (1664-302X) (Frontiers Media Sa), 2015-02 , Vol. 6 , N. 162 , P. 1-14
DOI 10.3389/fmicb.2015.00162
WOS© Times Cited 8
Keyword(s) cold seeps, colonization, deep-sea, symbiont, hydrothermal vents, wood falls

Reducing conditions with elevated sulfide and methane concentrations in ecosystems such as hydrothermal vents, cold seeps or organic falls, are suitable for chemosynthetic primary production. Understanding processes driving bacterial diversity, colonization and dispersal is of prime importance for deep-sea microbial ecology. This study provides a detailed characterization of bacterial assemblages colonizing plant-derived substrates using a standardized approach over a geographic area spanning the North-East Atlantic and Mediterranean. Wood and alfalfa substrates in colonization devices were deployed for different periods at 8 deep-sea chemosynthesis-based sites in four distinct geographic areas. Pyrosequencing of a fragment of the 16S rRNA-encoding gene was used to describe bacterial communities. Colonization occurred within the first 14 days. The diversity was higher in samples deployed for more than 289 days. After 289 days, no relation was observed between community richness and deployment duration, suggesting that diversity may have reached saturation sometime in between. Communities in long-term deployments were different, and their composition was mainly influenced by the geographical location where devices were deployed. Numerous sequences related to horizontally-transmitted chemosynthetic symbionts of metazoans were identified. Their potential status as free-living forms of these symbionts was evaluated based on sequence similarity with demonstrated symbionts. Results suggest that some free-living forms of metazoan symbionts or their close relatives, such as Epsilonproteobacteria associated with the shrimp Rimicans exoculata, are efficient colonizers of plant substrates at vents and seeps.

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Table 1 197 KB Open access
Table 2 199 KB Open access
Table 3 202 KB Open access
Table 4 194 KB Open access
Table 5 564 KB Open access
Data sheet 203 KB Open access
Image 1 309 KB Open access
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Image 3 15 MB Open access
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Szafranski Kamil M., Deschamps Philippe, Cunha Marina R., Gaudron Sylvie M., Duperron Sebastien (2015). Colonization of plant substrates at hydrothermal vents and cold seeps in the northeast Atlantic and Mediterranean and occurrence of symbiont-related bacteria. Frontiers In Microbiology, 6(162), 1-14. Publisher's official version : , Open Access version :