Donor and recipient contribution to phenotypic traits and the expression of biomineralisation genes in the pearl oyster model Pinctada margaritifera

Type Article
Date 2017-06
Language English
Author(s) Blay Carole1, 2, 3, Planes Serge2, 3, Ky Chin-Long1, 3
Affiliation(s) 1 : IFREMER, Ctr Pacifique, UMR EIO241, Labex Corail, BP 49, F-98719 Tahiti, French Polynesi, France.
2 : Univ Perpignan, PSL Res Univ, EPHE UPVD CNRS, USR CRIOBE 3278, 52 Ave Paul Alduy, F-66860 Perpignan, France.
3 : Lab Excellence CORAIL, 52 Ave Paul Alduy, F-66860 Perpignan, France.
Source Scientific Reports (2045-2322) (Nature Publishing Group), 2017-06 , Vol. 7 , N. 1 , P. 2696 (1-12)
DOI 10.1038/s41598-017-02457-x
WOS© Times Cited 11
Abstract

Grafting associates two distinct genotypes, each of which maintains its own genetic identity throughout the life of the grafted organism. Grafting technology is well documented in the plant kingdom, but much less so in animals. The pearl oyster, Pinctada margaritifera, produces valuable pearls as a result of the biomineralisation process of a mantle graft from a donor inserted together with a nucleus into the gonad of a recipient oyster. To explore the respective roles of donor and recipient in pearl formation, a uniform experimental graft was designed using donor and recipient oysters monitored for their growth traits. At the same time, phenotypic parameters corresponding to pearl size and quality traits were recorded. Phenotypic interaction analysis demonstrated: 1) a positive correlation between recipient shell biometric parameters and pearl size, 2) an individual donor effect on cultured pearl quality traits. Furthermore, the expressions of biomineralisation biomarkers encoding proteins in the aragonite or prismatic layer showed: 1) higher gene expression levels of aragonite-related genes in the large donor phenotype in the graft tissue, and 2) correlation of gene expression in the pearl sac tissue with pearl quality traits and recipient biometric parameters. These results emphasize that pearl size is mainly driven by the recipient and that pearl quality traits are mainly driven by the donor.

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