Rearing in captivity affects spermatogenesis and sperm quality in greater amberjack, (Risso, 1810)

Type Article
Date 2017-09
Language English
Author(s) Zupa R.1, Fauvel Christian2, Mylonas C. C.3, Pousis C.1, Santamaria N.1, Papadaki M.3, Fakriadis I.3, 4, Cicirelli V.1, Mangano S.5, Passantino L.1, Lacalandra G. M.1, Corriero Aldo1
Affiliation(s) 1 : Univ Bari Aldo Moro, Sect Vet Clin & Anim Prod, Dept Emergency & Organ Transplantat, I-70010 Bari, Italy.
2 : IRD UM2 CNRS IFREMER, Stn Ifremer, UMR MARBEC, F-34250 Palavas Les Flots, France.
3 : Hellen Ctr Marine Res, Inst Marine Biol Biotechnol & Aquaculture, Iraklion 71003, Crete, Greece.
4 : Univ Crete, Dept Biol, Iraklion 71003, Crete, Greece.
5 : CNR, Inst Marine Coastal Environm, I-91021 Capo Granitola, TP, Italy.
Source Journal Of Animal Science (0021-8812) (Amer Soc Animal Science), 2017-09 , Vol. 95 , N. 9 , P. 4085-4100
DOI 10.2527/jas2017.1708
WOS© Times Cited 1
Keyword(s) germ cell apoptosis, germ cell proliferation, greater amberjack, rearing in captivity, Seriola dumerili, sperm quality
Abstract

The greater amberjack, Seriola dumerili (Risso, 1810), is a promising candidate for the diversification of European aquaculture production, but inconsistent reproduction in captivity prevents commercial production. Recent studies showed that greater amberjack confined in sea cages exhibited scarce gonad development and early interruption of gametogenic activity during the reproductive season. The aim of the present study was to improve our understanding of the observed impairment of spermatogenesis. Adult wild and captive-reared males were sampled during 3 different phases of the reproductive cycle: early gametogenesis (EARLY; late April to early May), advanced gametogenesis (ADVANCED; late May to early June), and spawning (SPAWNING; late June to July). Spermatogonial stem cells and proliferating germ cells were identified through the immunohistochemical localization of Pou5f1 and proliferating cell nuclear antigen, respectively. Apoptotic germ cells were identified throughout the terminal deoxynucleotidyl transferase-mediated 2’-deoxyuridine 5’-triphosphate nick end labeling method. Sperm quality of captive-reared fish was evaluated using computer-assisted sperm analysis. Captive-reared males exhibited seminiferous lobules of a smaller diameter, a precocious and progressive decrease of spermatogonial mitosis, and a high level of apoptosis at the beginning of the reproductive season, concomitant with a many-fold higher 17β-estradiol plasma concentration. The motile spermatozoa percentage of captive greater amberjack was lower than in other teleosts, and a drastic decrease of spermatozoa motility duration, velocity, and ATP content occurred along the reproductive season. An abnormal increase of sperm concentration as well as an increase of dead spermatozoa occurred during the SPAWNING phase, probably because of lack of sperm hydration and ejaculation and consequent sperm ageing. The present study demonstrates the extreme susceptibility of greater amberjack to rearing stress and underscores the need for improvement of the rearing and handling procedures to ameliorate gametogenesis dysfunctions in commercial aquaculture production.

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Zupa R., Fauvel Christian, Mylonas C. C., Pousis C., Santamaria N., Papadaki M., Fakriadis I., Cicirelli V., Mangano S., Passantino L., Lacalandra G. M., Corriero Aldo (2017). Rearing in captivity affects spermatogenesis and sperm quality in greater amberjack, (Risso, 1810). Journal Of Animal Science, 95(9), 4085-4100. Publisher's official version : https://doi.org/10.2527/jas2017.1708 , Open Access version : https://archimer.ifremer.fr/doc/00395/50613/